Identification of Cancer Stem Cell-Related MicroRNAs in Hepatocellular Carcinoma

  • Junfang Ji
  • Xin Wei WangEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 826)


Cancer Stem cells (CSCs) are the source of many solid tumor types including hepatocellular carcinoma. MicroRNAs (miRNAs) are small noncoding RNAs and have been showed to be associated with hepatic CSCs. Here, we described methods to screen hepatic CSC-related miRNAs, and to validate and examine their expressions and functions in vitro and in vivo, which contribute to the maintenance of stemness and differentiation of hepatic CSCs.

Key words

Hepatic cancer stem cells MicroRNAs Hepatocellular carcinoma EpCAM Fluorescence-activated cell sorting MicroRNA microarray Quantitative reverse transcription-polymerase chain reaction Transfection Spheroid assay Tumorigenicity assay 



This work was supported by the Intramural Research Program of the Center for Cancer Research, the National Cancer Institute (Z01 BC 010313 and Z01 BC 010876).


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Laboratory of Human CarcinogenesisBethesdaUSA

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