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Astrocytes pp 251–263Cite as

Induction of Nitric Oxide Synthase-2 Expression and Measurement of Nitric Oxide Production in Enriched Primary Cortical Astrocyte Cultures

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 814))

Abstract

Astrocytes produce numerous mediators under conditions of inflammation in the central nervous system. One such mediator is nitric oxide (NO) derived from nitric oxide synthase-2 (NOS-2), the high output, inducible NOS isoform. Expression of NOS-2 and production of NO can be stimulated in astrocyte cultures by combinations of cytokines and lipopolysaccharide, a gram-negative bacterial endotoxin. This chapter details methods to induce and analyze NOS-2 expression and NO production in astrocyte cultures.

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Acknowledgments

The authors thank Tracy Uliasz and Nicole Jackman for providing cortical astrocyte cultures. This work was supported by NIH/NINDS grants 5R01NS036812 and 5R01NS051445.

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Authors and Affiliations

  1. Department of Biology, Syracuse University, Syracuse, NY, USA

    James A. Hewett & Sandra J. Hewett

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  1. James A. Hewett
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  2. Sandra J. Hewett
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Correspondence to James A. Hewett .

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Editors and Affiliations

  1. Dept. Molecular & Experimental, Scripps Research Institute, N. Torrey Pines Road 10550, La Jolla, 92037, California, USA

    Richard Milner

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Hewett, J.A., Hewett, S.J. (2012). Induction of Nitric Oxide Synthase-2 Expression and Measurement of Nitric Oxide Production in Enriched Primary Cortical Astrocyte Cultures. In: Milner, R. (eds) Astrocytes. Methods in Molecular Biology, vol 814. Humana Press. https://doi.org/10.1007/978-1-61779-452-0_17

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  • DOI: https://doi.org/10.1007/978-1-61779-452-0_17

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