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Innovative and Rapid Procedure for 4-Hydroxyproline Determination in Meat-Based Foods

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Amino Acid Analysis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 828))

Abstract

This report describes a rapid and innovative microwave procedure for protein hydrolysis coupled with high performance anion exchange chromatography and pulsed amperometric detection (HPAEC-PAD) to quantify the amino acid 4-hydroxyproline in meat and meat-based products. This innovative approach was successfully applied to determine collagen content (4-hydroxyproline × 8) as the index quality of meat material used in the preparation of typical meat-based foods.

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References

  1. Flores J (1980) Control de calidad de los productos carnicos. Rev Agroquim Tecnol Aliment 20:180

    Google Scholar 

  2. Vazquez-Ortiz FA, Gonzalez-Mendez NF (1996) Determination of collagen as a quality index in Bologna from Northwestern Mexico. J Food Comp Anal 9:269–276

    Article  CAS  Google Scholar 

  3. Etherington DJ, Trevor SJ (1981) Detection and estimation of collagen. J Sci Food Agric 32:539–546

    Article  CAS  Google Scholar 

  4. Chiou SH, Wang KT (1989) Peptide and protein hydrolysis by microwave irradiation. J Chromatogr 491:424–431

    Article  PubMed  CAS  Google Scholar 

  5. Fountoulakis M, Lahm HW (1998) Hydrolysis and amino acid composition analysis of proteins. J Chromatogr A 826:109–134

    Article  PubMed  CAS  Google Scholar 

  6. Huan YF et al (2009) PPESO3 monitoring the process of microwave enhanced enzymatic digestion on protein. Chem J Chinese Univ 30(1):54–56

    CAS  Google Scholar 

  7. Carisano A (1993) Digestione delle proteine in matrici alimentari con forno a microonde. 2-determinazione del triptofano. Ind Aliment 32:346–349

    CAS  Google Scholar 

  8. Marconi E et al (1996) Fast analysis of lysine in food using protein microwave hydrolysis and an electrochemical biosensor. Anal Lett 29 (7):1125–1137

    Article  CAS  Google Scholar 

  9. Marconi E, Panfili G, Acquistucci R (1997) Microwave hydrolysis for the rapid analysis of furosine in foods. Italian J Food Sci 1 (9):47–55

    Google Scholar 

  10. Marconi E, Mastrocola L, Panfili G (1999) Improvement of microwave hydrolysis for fast analysis of furosine in milk and dairy products. Sci Tec Lattiero-Casearia 50(2):127–137

    CAS  Google Scholar 

  11. Marconi E et al (2000) Rapid detection of meso-diaminopimelic acid in lactic acid bacteria by microwave hydrolysis. J Agric Food Chem 48(8):3348–3351

    Article  PubMed  CAS  Google Scholar 

  12. Chen ST et al (1987) Rapid hydrolysis of proteins and peptides by means of microwave technology and its application to amino acid analysis. Int J Pept Protein Res 30:572–576

    Article  PubMed  Google Scholar 

  13. Gilman LB, Woodward C (1990) An evaluation of microwave heating for vapour phase hydrolysis of proteins. Comparison to vapour phase hydrolysis for 24 hours. In Gilman LB (ed) Current research in protein Chemistry, Academic Press: New York, NY

    Google Scholar 

  14. Woodward C, Gilman LB, Engelhart WG (1990) An evaluation of microwave heating for vapour phase hydrolysis of proteins. Int Lab 20:40–46

    Google Scholar 

  15. Marconi E et al (1995) Comparative study on microwave and conventional methods for protein hydrolysis in food. Amino Acids 8:201–208

    Article  CAS  Google Scholar 

  16. Spackman DH, Stein WH, Moore S (1958) Chromatography of amino acids on sulphonated resins. Anal Chem 30:1190–1196

    Article  CAS  Google Scholar 

  17. Polta JA, Johnson DC (1983) The direct electrochemical detection of amino-acids at platinum electrode in an alkaline chromatographic effluent. J Liq Chromatogr 6:1727–1743

    Article  CAS  Google Scholar 

  18. Marten DA, Frankenberg WT (1992) Pulsed amperometric detection of amino-acids separated by anion-exchange chromatography. J Liq Chromatogr 15:423–439

    Article  Google Scholar 

  19. Welch LE et al (1989) Comparison of pulsed coulometric detection and potential-sweep pulsed coulometric detection for underivatized amino-acids in liquid chromatography. Anal Chem 61:555–559

    Article  PubMed  CAS  Google Scholar 

  20. Clarke AP et al (1999) An integrated amperometry waveform for the direct, sensitive detection of amino acids and amino sugars following anion-exchange chromatography. Anal Chem 71:2774–2781

    Article  CAS  Google Scholar 

  21. Messia MC et al (2008) Rapid determination of collagen in meat based foods by microwave hydrolysis and HPAEC-PAD analysis of 4-hydroxyproline. Meat Sci 80:401–409

    Article  PubMed  CAS  Google Scholar 

  22. AOAC (2000). OFFICIAL METHOD 990.26, and 928.08, Official Methods of Analysis, 17th edn. Gaithersburg, MD; Association of Official Analytical Chemists.

    Google Scholar 

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Correspondence to Emanuele Marconi .

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Messia, M.C., Marconi, E. (2012). Innovative and Rapid Procedure for 4-Hydroxyproline Determination in Meat-Based Foods. In: Alterman, M., Hunziker, P. (eds) Amino Acid Analysis. Methods in Molecular Biology, vol 828. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-445-2_22

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  • DOI: https://doi.org/10.1007/978-1-61779-445-2_22

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-444-5

  • Online ISBN: 978-1-61779-445-2

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