Abstract
This report describes a rapid and innovative microwave procedure for protein hydrolysis coupled with high performance anion exchange chromatography and pulsed amperometric detection (HPAEC-PAD) to quantify the amino acid 4-hydroxyproline in meat and meat-based products. This innovative approach was successfully applied to determine collagen content (4-hydroxyproline × 8) as the index quality of meat material used in the preparation of typical meat-based foods.
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References
Flores J (1980) Control de calidad de los productos carnicos. Rev Agroquim Tecnol Aliment 20:180
Vazquez-Ortiz FA, Gonzalez-Mendez NF (1996) Determination of collagen as a quality index in Bologna from Northwestern Mexico. J Food Comp Anal 9:269–276
Etherington DJ, Trevor SJ (1981) Detection and estimation of collagen. J Sci Food Agric 32:539–546
Chiou SH, Wang KT (1989) Peptide and protein hydrolysis by microwave irradiation. J Chromatogr 491:424–431
Fountoulakis M, Lahm HW (1998) Hydrolysis and amino acid composition analysis of proteins. J Chromatogr A 826:109–134
Huan YF et al (2009) PPESO3 monitoring the process of microwave enhanced enzymatic digestion on protein. Chem J Chinese Univ 30(1):54–56
Carisano A (1993) Digestione delle proteine in matrici alimentari con forno a microonde. 2-determinazione del triptofano. Ind Aliment 32:346–349
Marconi E et al (1996) Fast analysis of lysine in food using protein microwave hydrolysis and an electrochemical biosensor. Anal Lett 29 (7):1125–1137
Marconi E, Panfili G, Acquistucci R (1997) Microwave hydrolysis for the rapid analysis of furosine in foods. Italian J Food Sci 1 (9):47–55
Marconi E, Mastrocola L, Panfili G (1999) Improvement of microwave hydrolysis for fast analysis of furosine in milk and dairy products. Sci Tec Lattiero-Casearia 50(2):127–137
Marconi E et al (2000) Rapid detection of meso-diaminopimelic acid in lactic acid bacteria by microwave hydrolysis. J Agric Food Chem 48(8):3348–3351
Chen ST et al (1987) Rapid hydrolysis of proteins and peptides by means of microwave technology and its application to amino acid analysis. Int J Pept Protein Res 30:572–576
Gilman LB, Woodward C (1990) An evaluation of microwave heating for vapour phase hydrolysis of proteins. Comparison to vapour phase hydrolysis for 24 hours. In Gilman LB (ed) Current research in protein Chemistry, Academic Press: New York, NY
Woodward C, Gilman LB, Engelhart WG (1990) An evaluation of microwave heating for vapour phase hydrolysis of proteins. Int Lab 20:40–46
Marconi E et al (1995) Comparative study on microwave and conventional methods for protein hydrolysis in food. Amino Acids 8:201–208
Spackman DH, Stein WH, Moore S (1958) Chromatography of amino acids on sulphonated resins. Anal Chem 30:1190–1196
Polta JA, Johnson DC (1983) The direct electrochemical detection of amino-acids at platinum electrode in an alkaline chromatographic effluent. J Liq Chromatogr 6:1727–1743
Marten DA, Frankenberg WT (1992) Pulsed amperometric detection of amino-acids separated by anion-exchange chromatography. J Liq Chromatogr 15:423–439
Welch LE et al (1989) Comparison of pulsed coulometric detection and potential-sweep pulsed coulometric detection for underivatized amino-acids in liquid chromatography. Anal Chem 61:555–559
Clarke AP et al (1999) An integrated amperometry waveform for the direct, sensitive detection of amino acids and amino sugars following anion-exchange chromatography. Anal Chem 71:2774–2781
Messia MC et al (2008) Rapid determination of collagen in meat based foods by microwave hydrolysis and HPAEC-PAD analysis of 4-hydroxyproline. Meat Sci 80:401–409
AOAC (2000). OFFICIAL METHOD 990.26, and 928.08, Official Methods of Analysis, 17th edn. Gaithersburg, MD; Association of Official Analytical Chemists.
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Messia, M.C., Marconi, E. (2012). Innovative and Rapid Procedure for 4-Hydroxyproline Determination in Meat-Based Foods. In: Alterman, M., Hunziker, P. (eds) Amino Acid Analysis. Methods in Molecular Biology, vol 828. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-445-2_22
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DOI: https://doi.org/10.1007/978-1-61779-445-2_22
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