Rho GTPases pp 87-95 | Cite as

Posttranslational Lipid Modification of Rho Family Small GTPases

  • Natalia MitinEmail author
  • Patrick J. Roberts
  • Emily J. Chenette
  • Channing J. Der
Part of the Methods in Molecular Biology book series (MIMB, volume 827)


The Rho family comprises a major branch of the Ras superfamily of small GTPases. A majority of Rho GTPases are synthesized as inactive, cytosolic proteins. They then undergo posttranslational modification by isoprenoid or fatty acid lipids, and together with additional carboxyl-terminal sequences target Rho GTPases to specific membrane and subcellular compartments essential for function. We summarize the use of biochemical and cellular assays and pharmacologic inhibitors instrumental for the study of the role of posttranslational lipid modifications and processing in Rho GTPase biology.

Key words

CAAX motif Farnesylation Geranylgeranylation Palmitoylation Ras-converting enzyme 1 Isoprenylcysteine carboxyl methyltransferase 



This work was supported, in whole or in part, by National Institutes of Health Grants CA063071, CA67771, and CA92240 to C.J.D.


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Natalia Mitin
    • 1
    Email author
  • Patrick J. Roberts
    • 1
  • Emily J. Chenette
    • 1
  • Channing J. Der
    • 2
  1. 1.Lineberger Comprehensive Cancer CenterUniversity of North CarolinaChapel HillUSA
  2. 2.Lineberger Comprehensive Cancer Center and Department of PharmacologyUniversity of North CarolinaChapel HillUSA

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