Abstract
Rho proteins act as molecular switches to control multiple cellular processes. The switch mechanism involves cycling between active and inactive states based on GTP loading and hydrolysis. Assays that quantitatively analyze the GTP loading of Rho proteins have become important molecular tools to decipher upstream signals and mechanisms that regulate activation and de-activation. These assays make use of Rho activation probes constructed from Rho-binding domains of downstream effectors. The utility of these assays comes from effector domains that show selective high affinity interactions with specific subsets of GTP-bound activated GTPases. Here, we describe assays used to analyze yeast Rho GTPase activation.
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Acknowledgments
We would like to acknowledge the Canadian Institutes of Health Research and the National Science and Engineering Research Council of Canada for funding projects that study Rho GTPases in yeast.
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Eitzen, G., Logan, M.R. (2012). Analysis of Rho GTPase Activation in Saccharomyces cerevisiae . In: Rivero, F. (eds) Rho GTPases. Methods in Molecular Biology, vol 827. Springer, New York, NY. https://doi.org/10.1007/978-1-61779-442-1_24
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DOI: https://doi.org/10.1007/978-1-61779-442-1_24
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