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Control of the Interferon Response in RNAi Experiments

  • Jana Nejepinska
  • Matyas Flemr
  • Petr SvobodaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 820)

Abstract

The RNA interference (RNAi) and interferons have been an uneasy marriage. Ever since the discovery of RNAi in mammals, the interferon response has been a feared problem. While RNAi became an efficient and widespread method for gene silencing in mammals, numerous studies recognized several obstacles, including undesirable activation of the interferon response, which need to be overcome to achieve a specific and robust RNAi effect. The aim of this text is to provide theoretical and practical information for scientists who want to control interferon response and other adverse effects in their RNAi experiments.

Key words

RNA interference Small interfering RNA Short hairpin RNA Double-stranded RNA Interferon 

Notes

Acknowledgements

We thank Witold Filipowicz group at the FMI for sharing their experience and protocols and Daniela Schmitter, Radek Malik, and Lenka Sarnova for help with preparation of the manuscript. Protocols are based on research supported by the GAAV grant IAA 501110701, EMBO SDIG grant 2006–1483, GACR grant 204/09/0085, and the Purkynje Fellowship.

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© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Institute of Molecular Genetics, Academy of Sciences of the Czech RepublicPrague 4Czech Republic

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