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Integrin-Targeted Stabilized Nanoparticles for an Efficient Delivery of siRNAs In Vitro and In Vivo

  • Charudharshini Srinivasan
  • Dan Peer
  • Motomu ShimaokaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 820)

Abstract

Utilizing small interfering RNAs (siRNAs) to silence disease-associated genes holds promise as a potential therapeutic strategy. However, the greatest challenge for RNAi remains the delivery of siRNA to target tissues or cells. Specifically lymphocytes are difficult to transduce by conventional methods but represent good targets for anti-inflammatory therapeutics. Integrins are an important class of cell adhesion receptors on leukocytes. Antibodies to integrins have been used to inhibit inflammatory reactions in patients. Here, we describe a strategy to deliver the siRNA cargo to leukocytes by stabilized nanoparticles surface-decorated with antibodies to integrin as targeting moieties. A detailed methodology for preparation of the integrin-targeted stabilized nanoparticles (I-tsNPs) and their delivery in vitro and in vivo is discussed.

Key words

Liposomes RNAi Leukocytes Inflammation Hyaluronan Antibody Transfection Systemic delivery 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Charudharshini Srinivasan
    • 1
    • 2
    • 3
  • Dan Peer
    • 4
  • Motomu Shimaoka
    • 1
    • 2
    • 3
    Email author
  1. 1.Immune Disease InstituteBostonUSA
  2. 2.Program in Cellular and Molecular Medicine, Children’s Hospital BostonBostonUSA
  3. 3.Department of AnesthesiaHarvard Medical SchoolBostonUSA
  4. 4.Laboratory of Nanomedicine, Department of Cell Research & Immunology, George S. Wise Faculty of Life Sciences, and the Center for Nanoscience and NanotechnologyTel Aviv UniversityTel AvivIsrael

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