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Ligand Affinity Chromatography, an Indispensable Method for the Purification of Soluble Cytokine Receptors and Binding Proteins

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Book cover Cytokine Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 820))

Abstract

Ligand affinity chromatography separation is based on unique interaction between the target analyte and a ligand, which is coupled covalently to a resin. It is a simple, rapid, selective, and efficient purification procedure of proteins providing tens of thousands fold purification in one step. The biological activity of the isolated proteins is retained in most cases thus function is revealed concomitantly with the isolation. Prior to the completion of the genome project this method facilitated rapid and reliable cloning of the corresponding gene. Upon completion of this project, a partial protein sequence is enough for retrieving its complete mRNA and hence its complete protein sequence. This method is indispensable for the isolation of both expected (e.g. receptors) but mainly unexpected, unpredicted and very much surprising binding proteins. No other approach would yield the latter. This chapter provides examples for both the expected target proteins, isolated from rich sources of human proteins, as well as the unexpected binding proteins, found by serendipity.

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Authors and Affiliations

  1. Department of Molecular Genetics, The Weizmann Institute of Science, Rehovot, 76100, Israel

    Daniela Novick & Menachem Rubinstein

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  1. Daniela Novick
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  2. Menachem Rubinstein
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Correspondence to Daniela Novick .

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Editors and Affiliations

  1. Afd. Biochemie, Katholieke Universiteit Leuven, Celestijnenlaan 200 G, Leuven, 3001, Belgium

    Marc De Ley

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Novick, D., Rubinstein, M. (2012). Ligand Affinity Chromatography, an Indispensable Method for the Purification of Soluble Cytokine Receptors and Binding Proteins. In: De Ley, M. (eds) Cytokine Protocols. Methods in Molecular Biology, vol 820. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-439-1_12

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  • DOI: https://doi.org/10.1007/978-1-61779-439-1_12

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-438-4

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