shRNA-Induced Interferon-Stimulated Gene Analysis

  • Núria MorralEmail author
  • Scott R. Witting
Part of the Methods in Molecular Biology book series (MIMB, volume 820)


RNA interference (RNAi) is a cellular mechanism to inhibit the expression of gene products in a highly specific manner. In recent years, RNAi has become the cornerstone of gene function studies, shortening the otherwise long process of target identification and validation. In addition, small interfering RNA (siRNA) and short-hairpin RNA (shRNA) therapies are being developed for the treatment of a variety of human diseases. Despite its huge potential for gene silencing, a hurdle to safe and effective RNAi is the activation of innate immune responses. Induction of innate immunity is dose- and sequence-dependent, and is also influenced by target tissue and delivery vehicle. Research on the molecular mechanisms mediating this response is helping to improve the design of the RNAi molecules. Nevertheless, appropriate testing for the presence of this undesired effect is needed prior to making conclusions on the outcome of the silencing treatment.

Key words

RNA interference Short-hairpin RNA Gene transfer Animal models Interferon response Interferon-stimulated gene 



This research was supported by grants from the NIDDK (DK069432-01 and DK078595), American Diabetes Foundation (1-08-RA-135), and INGEN (Indiana Genomics Initiative of Indiana University supported in part by Lilly Endowment Inc.).


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© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of Medical and Molecular Genetics, and Center for Diabetes ResearchIndiana University School of MedicineIndianapolisUSA

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