Abstract
N-alpha protein acetylation, catalyzed by N-alpha acetyltransferase complex, is a common protein modification process in eukaryotic cells. Despite its widespread occurrence, the biological significance of this modification process is still unclear. We recently discovered a novel testis-predominant isoform of the catalytic subunit of the enzyme complex. Here, we describe the biochemical characterization of this testis-predominant N-alpha acetyltransferase complex, which includes protein–protein interaction study by co-immunoprecipitation experiment and functional study by N-alpha acetyltransferase assay.
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Acknowledgments
We would like to thank Roderick A. Corriveau (Department of Cell Biology and Anatomy, Louisiana State University) for the expression vectors CS2 + MT-mNAT1, CS2 + mARD1/HA, pT7-mNAT1, and pBSARD1/HA. This work was supported by the Intramural Research Program of Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health.
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Pang, A.LY. (2012). Biochemical Characterization of a Testis-Predominant Isoform of N-Alpha Acetyltransferase. In: Chan, WY., Blomberg, L. (eds) Germline Development. Methods in Molecular Biology, vol 825. Springer, New York, NY. https://doi.org/10.1007/978-1-61779-436-0_8
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DOI: https://doi.org/10.1007/978-1-61779-436-0_8
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