Abstract
The mammalian Target of Rapamycin (mTOR) defines a crucial link between nutrient sensing and immune function. In CD4+ T cells, mTOR has been shown to play a critical role in regulating effector and regulatory T cell differentiation as well as the decision between full activation versus the induction of anergy. In this chapter, we describe how our group has employed the Cre-lox technology to genetically delete components of the mTOR signaling complex in T cells. This has enabled us to specifically interrogate mTOR function in T cells both in vitro and in vivo. We also describe techniques used to assay immune function and signaling in mTOR-deficient T cells at the single-cell level.
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Acknowledgments
The authors would like to thank members of the Powell laboratory for technical assistance in optimizing these models. In addition, we would like to thank Dr. Sara C. Kozma (U. Cincinnati) for generating the original floxed mouse lines. This work was supported by R01AI077610-01A2.
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Delgoffe, G.M., Powell, J.D. (2012). Exploring Functional In Vivo Consequences of the Selective Genetic Ablation of mTOR Signaling in T Helper Lymphocytes. In: Weichhart, T. (eds) mTOR. Methods in Molecular Biology, vol 821. Humana Press. https://doi.org/10.1007/978-1-61779-430-8_20
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DOI: https://doi.org/10.1007/978-1-61779-430-8_20
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