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Introduction to miRNA Profiling Technologies and Cross-Platform Comparison

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Next-Generation MicroRNA Expression Profiling Technology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 822))

Abstract

MicroRNA analysis has been widely adopted for basic and applied science. The tools and technologies available for quantifying and analysing miRNAs are still maturing. Here, we give an introductory overview of the main tools and the challenges in their use. We also discuss the importance of basic experimental design, sample handling and analysis methods as the impact of these can be as profound as the choice of miRNA analysis platform. Whether the reader is interested in a gene-by-gene or genome-wide approach choosing the platform to use is not trivial. Careful thought given before starting an experiment will make the execution much easier.

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Acknowledgements

We thank Stefan Graf, Heidi Dvinge, Claudia Kutter, and Anna Git for their helpful comments on the manuscript.

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  1. CRUK Cambridge Research Institute, Cambridge, UK

    Sarah Aldridge & James Hadfield

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  1. Sarah Aldridge
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  2. James Hadfield
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Correspondence to James Hadfield .

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  1. Illumina, Inc., Towne Centre Drive 9885, San Diego, 92121, California, USA

    Jian-Bing Fan

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Aldridge, S., Hadfield, J. (2012). Introduction to miRNA Profiling Technologies and Cross-Platform Comparison. In: Fan, JB. (eds) Next-Generation MicroRNA Expression Profiling Technology. Methods in Molecular Biology, vol 822. Humana Press. https://doi.org/10.1007/978-1-61779-427-8_2

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  • DOI: https://doi.org/10.1007/978-1-61779-427-8_2

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-426-1

  • Online ISBN: 978-1-61779-427-8

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