Genome-Wide Screens for Expressed Hypothetical Proteins

  • Claus DeslerEmail author
  • Jon Ambæk Durhuus
  • Lene Juel Rasmussen
Part of the Methods in Molecular Biology book series (MIMB, volume 815)


A hypothetical protein (HP) is defined as a protein that is predicted to be expressed from an open reading frame, but for which there is no experimental evidence of translation. HPs constitute a substantial fraction of proteomes of human as well as of other organisms. With the general belief that the majority of HPs are the product of pseudogenes, it is essential to have a tool with the ability of pinpointing the minority of HPs with a high probability of being expressed.

Key words

Hypothetical proteins In silico Pseudogenes 



This work was supported by a grant from the NORDEA foundation.


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Claus Desler
    • 1
    Email author
  • Jon Ambæk Durhuus
    • 2
  • Lene Juel Rasmussen
    • 2
  1. 1.Center for Healthy AgingUniversity of CopenhagenCopenhagenDenmark
  2. 2.Center for Healthy Aging, Faculty of Health SciencesUniversity of CopenhagenCopenhagenDenmark

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