Metabolite Analysis of Cannabis sativa L. by NMR Spectroscopy

  • Isvett Josefina Flores-SanchezEmail author
  • Young Hae Choi
  • Robert Verpoorte
Part of the Methods in Molecular Biology book series (MIMB, volume 815)


NMR-based metabolomics is an analytical platform, which has been used to classify and analyze Cannabis sativa L. cell suspension cultures and plants. Diverse groups of primary and secondary metabolites were identified by comparing NMR data with reference compounds and/or by structure elucidation using 1H-NMR, J-resolved, 1H-1H COSY, and 1H-13C HMBC spectroscopy. The direct extraction and the extraction by indirect fractionation are two suitable methods for the C. sativa sample preparation. Quantitative analyses could be performed without requiring fractionation or isolation procedures.

Key words

Cannabis sativa Metabolomics Multivariate data analysis Nuclear magnetic resonance Principal component analysis 


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Isvett Josefina Flores-Sanchez
    • 1
    Email author
  • Young Hae Choi
    • 2
  • Robert Verpoorte
    • 2
  1. 1.Institute of Biological ChemistryWashington State UniversityPullmanUSA
  2. 2.Pharmacognosy Department/Metabolomics, Gorlaeus Laboratories, Institute of BiologyLeiden UniversityRA LeidenThe Netherlands

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