Yeast Two-Hybrid Screens: Improvement of Array-Based Screening Results by N- and C-terminally Tagged Fusion Proteins
Matrix-based yeast two-hybrid screens are an alternative to library-based screens. Recent improvements of matrix screens (also called array screens), use various pooling strategies as well as novel vectors that increase its efficiency while decreasing the false-negative rate, thus increasing reliability. In this chapter, we describe a screening strategy that systematically combines N- and C-terminal fusion proteins using a recently developed vector system.
Key wordsYeast two-hybrid Protein–protein interactions Permutated fusion tags
This work was supported by the German Government via BMBF (Zoonosis Network, Consortium on ecology and pathogenesis of SARS, project code 01KIO701) to A.v.B, by a grant of the Baden-Württemberg Stiftung (Germany) to P.U., R.H. and T.S., and by a grant from the European Union (HEALTH-F3-2009-223101) to P.U.
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