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Yeast Two-Hybrid Screens: Improvement of Array-Based Screening Results by N- and C-terminally Tagged Fusion Proteins

  • Thorsten Stellberger
  • Roman Häuser
  • Peter Uetz
  • Albrecht von BrunnEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 815)

Abstract

Matrix-based yeast two-hybrid screens are an alternative to library-based screens. Recent improvements of matrix screens (also called array screens), use various pooling strategies as well as novel vectors that increase its efficiency while decreasing the false-negative rate, thus increasing reliability. In this chapter, we describe a screening strategy that systematically combines N- and C-terminal fusion proteins using a recently developed vector system.

Key words

Yeast two-hybrid Protein–protein interactions Permutated fusion tags 

Notes

Acknowledgments

This work was supported by the German Government via BMBF (Zoonosis Network, Consortium on ecology and pathogenesis of SARS, project code 01KIO701) to A.v.B, by a grant of the Baden-Württemberg Stiftung (Germany) to P.U., R.H. and T.S., and by a grant from the European Union (HEALTH-F3-2009-223101) to P.U.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Thorsten Stellberger
    • 1
  • Roman Häuser
    • 2
  • Peter Uetz
    • 3
  • Albrecht von Brunn
    • 4
    Email author
  1. 1.Max-von-Pettenkofer-Institut, Lehrstuhl VirologieLudwig-Maximilians-Universität (LMU)MünchenGermany
  2. 2.Institute of Toxicology and Genetics, Karlsruhe Institute of Technology (KIT)Eggenstein-LeopoldshafenGermany
  3. 3.Center for the Study of Biological ComplexityVirginia Commonwealth UniversityRichmondUSA
  4. 4.Max-von-Pettenkofer-Institut, Lehrstuhl VirologieLudwig-Maximilians-Universität (LMU)MünchenGermany

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