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Bioluminescence Resonance Energy Transfer: An Emerging Tool for the Detection of Protein–Protein Interaction in Living Cells

  • Søren W. GerstingEmail author
  • Amelie S. Lotz-Havla
  • Ania C. Muntau
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 815)

Abstract

In the field of proteomics, numerous advanced technologies have evolved that aim to provide the molecular data necessary for an in-depth understanding of biological processes. Protein–protein interactions (PPI) are at the heart of cellular function and a milestone yet to be achieved is the mapping of a complete human interactome. Bioluminescence resonance energy transfer (BRET) has become a popular technique to investigate PPI. As BRET enables the detection of PPI in living cells, problems associated with in vitro biochemical assays can be circumvented, thus making BRET a powerful tool for monitoring interactions of virtually all kinds of protein species.

Key words

Bioluminescence resonance energy transfer Protein–protein interaction Living cells Renilla luciferase Yellow fluorescent protein Nucleofection 

Notes

Acknowledgments

Financial support from the Bavarian Genome Research Network (BayGene) and the LMUexcellent grant 42595-6 to A.C.M. is gratefully acknowledged.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Søren W. Gersting
    • 1
    Email author
  • Amelie S. Lotz-Havla
    • 1
  • Ania C. Muntau
    • 1
  1. 1.Department of Molecular Pediatrics, Dr. von Hauner Children’s HospitalLudwig-Maximilians-UniversityMünichGermany

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