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The Use of Antigen Microarrays in Antibody Profiling

  • Krisztián Papp
  • József PrechlEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 815)

Abstract

Technological advances in the field of microarray production and analysis lead to the development of protein microarrays. Of these, antigen microarrays are one particular format that allows the study of antigen–antibody interactions in a miniaturized and highly multiplexed fashion. Here, we describe the parallel detection of antibodies with different specificities in human serum, a procedure also called antibody profiling. Autoantigens printed on microarray slides are reacted with test sera and the bound antibodies are identified by fluorescently labeled secondary reagents. Reactivity patterns generated this way characterize individuals and can help design novel diagnostic tools.

Key words

Protein microarray Antibody Profiling Immunoglobulin Immunity Immunoassay Antigen 

Notes

Acknowledgments

This work was supported by the Hungarian Academy of Sciences, grant KMOP-1.1.1-08/1-2008-0028 from the National Develop­ment Agency and NKTH-OTKA grant K68617. K.P. is supported by Janos Bolyai Research Fellowship. We thank Zoltán Szittner and Mariann Kremlitzka for their critical comments.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Immunology Research Group, ELTE-MTABudapestHungary

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