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Whole-Genome RT-qPCR MicroRNA Expression Profiling

  • Pieter Mestdagh
  • Stefaan Derveaux
  • Jo VandesompeleEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 815)

Abstract

MicroRNAs (miRNAs) are small noncoding RNA molecules that function as negative regulators of gene expression. They are essential components of virtually every biological process and deregulated miRNA expression has been reported in a multitude of human diseases including cancer. Owing to their small size (20–22 nucleotides), accurate quantification of miRNA expression is particularly challenging. In this chapter, we present different RT-qPCR technologies that enable whole genome miRNA expression quantification.

Key words

microRNA Stem-loop RT-qPCR Global mean normalization 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Pieter Mestdagh
    • 1
  • Stefaan Derveaux
    • 1
  • Jo Vandesompele
    • 1
    Email author
  1. 1.Center for Medical GeneticsGhent UniversityGhentBelgium

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