Abstract
We describe the culture and use of MLO-Y4 cells in studies of gene expression, response to fluid flow, and dendrite growth. We also describe how to use the MLO-A5 cells as a model of osteoblast to osteocyte differentiation and how to study their mineralization. These studies serve as a beginning point to study osteocyte functions and molecular mechanisms responsible for these functions.
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Rosser, J., Bonewald, L.F. (2012). Studying Osteocyte Function Using the Cell Lines MLO-Y4 and MLO-A5. In: Helfrich, M., Ralston, S. (eds) Bone Research Protocols. Methods in Molecular Biology, vol 816. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-415-5_6
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DOI: https://doi.org/10.1007/978-1-61779-415-5_6
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-414-8
Online ISBN: 978-1-61779-415-5
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