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Studying Gene Expression in Bone by In Situ Hybridization

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Bone Research Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 816))

Abstract

Here, we described a method for carrying out nonradioactive in situ hybridization to detect mRNA transcripts in cryostat sections of mouse bone using the CryoJane® Tape-Transfer System and digoxigenin (DIG)-labeled riboprobes.

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References

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Acknowledgments

We thank Dr. M. John (Novartis Institutes for BioMedical Research, Basel, Switzerland) and Prof. E. Schipani (Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA) for providing the DNA constructs for generation of type 1 collagen alpha 1 and type 10 collagen riboprobes. We are grateful to Profs. D. W. Rowe and I. Kalajzic (University of Connecticut Health Center, Farmington, CT, USA) as well as Prof. S. E. Harris (University of Texas Health Science Center at San Antonio, San Antonio, TX, USA) for advice on fixation and decalcification times, as well as cryo-sectioning with the CryoJane® Tape-Transfer System and ISH on skeletal tissue.

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Authors and Affiliations

  1. Musculoskeletal Disease Department, Novartis Institutes for BioMedical Research, Basel, Switzerland

    Ina Kramer, Rishard Salie & Michaela Kneissel

  2. Oncology Department, Novartis Institutes for BioMedical Research, Basel, Switzerland

    Mira Susa

Authors
  1. Ina Kramer
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  2. Rishard Salie
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  3. Mira Susa
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  4. Michaela Kneissel
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Corresponding author

Correspondence to Ina Kramer .

Editor information

Editors and Affiliations

  1. Institute of Medical Sciences, University of Aberdeen, Forester Hill, Aberdeen, AB25 2ZD, United Kingdom

    Miep H. Helfrich

  2. Institute of Genetics and Molecular Medi, Rheumatic Diseases Unit, University of Edinburgh, Crewe Road South, Edinburgh, EH4 2XU, United Kingdom

    Stuart H. Ralston

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© 2012 Springer Science+Business Media, LLC

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Kramer, I., Salie, R., Susa, M., Kneissel, M. (2012). Studying Gene Expression in Bone by In Situ Hybridization. In: Helfrich, M., Ralston, S. (eds) Bone Research Protocols. Methods in Molecular Biology, vol 816. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-415-5_20

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  • DOI: https://doi.org/10.1007/978-1-61779-415-5_20

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-414-8

  • Online ISBN: 978-1-61779-415-5

  • eBook Packages: Springer Protocols

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