Abstract
There is a large interest in the use of nucleic acids covalently bound to surfaces for a variety of biomedical uses: biosensors, microarrays, drug delivery, lab-on-chip devices, and gene therapy, etc. Most of these applications require the covalent attachment of oligonucleotides via specific reactive groups on both modified oligonucleotide and/or surface. The purpose of this chapter is to provide experimental protocols for the synthesis of oligonucleotides and for the immobilization of these synthetic oligonucleotides onto surfaces such as gold and silicon oxide.
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Acknowledgments
This work is supported by Spanish Ministry of Education (NAN2004-09415-C05-03, BFU2007-63287/BMC), Generalitat de Catalunya (2005/SGR/00693), Instituto de Salud Carlos III (CIBER-BNN), and European Communities (NANO-3D NMP4-CT2005-014006, DYNAMO contract 028669 (NEST), FUNMOL NMP4-SL-2009-213382). B.M. thanks the SFI-CRANN/UCD for the predoctoral fellowship. We are thankful to Elena Martínez and Xavier Sisquella of Scientific Park of Barcelona (PCB) for providing us template stripped gold.
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Manning, B., Eritja, R. (2012). Functionalization of Surfaces with Synthetic Oligonucleotides. In: Navarro, M., Planell, J. (eds) Nanotechnology in Regenerative Medicine. Methods in Molecular Biology, vol 811. Humana Press. https://doi.org/10.1007/978-1-61779-388-2_6
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DOI: https://doi.org/10.1007/978-1-61779-388-2_6
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