Micro/Nanopatterning of Proteins Using a Nanoimprint-Based Contact Printing Technique
Micro and nanoscale protein patterning based on microcontact printing technique on large substrates have often resolution problems due to roof collapse of the poly(dimethylsiloxane) (PDMS) stamps used. Here, we describe a technique that overcomes these issues by using instead a stamp made of poly(methyl methacrylate) (PMMA), a much more rigid polymer that do not collapse even using stamps with very high aspect ratios (up to 300:1). Conformal contact between the stamp and the substrate is achieved because of the homogeneous pressure applied via the nanoimprint lithography instrument, and it has allowed us to print lines of protein 150 nm wide, at a 400 nm period. This technique, therefore, provides an excellent method for the direct printing of high-density submicrometer scale patterns, or, alternatively, micro/nanopatterns spaced at large distances.
Key wordsMicrocontact printing Protein Poly(methyl methacrylate) Nanoimprint lithography
E.M. is grateful to the Spanish Ministry of Science and Education for the provision of grants through the I3 system. We acknowledge financial support from the Spanish Ministerio de Ciencia e Innovación through the project entitled “Regenerative Stem Cell Therapies for Heart Failure” and from the Science Support Program of the Fundación Marcelino Botín.