Advertisement

Micro/Nanopatterning of Proteins Using a Nanoimprint-Based Contact Printing Technique

  • Elena MartínezEmail author
  • Mateu Pla-Roca
  • Josep Samitier
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 811)

Abstract

Micro and nanoscale protein patterning based on microcontact printing technique on large substrates have often resolution problems due to roof collapse of the poly(dimethylsiloxane) (PDMS) stamps used. Here, we describe a technique that overcomes these issues by using instead a stamp made of poly(methyl methacrylate) (PMMA), a much more rigid polymer that do not collapse even using stamps with very high aspect ratios (up to 300:1). Conformal contact between the stamp and the substrate is achieved because of the homogeneous pressure applied via the nanoimprint lithography instrument, and it has allowed us to print lines of protein 150 nm wide, at a 400 nm period. This technique, therefore, provides an excellent method for the direct printing of high-density submicrometer scale patterns, or, alternatively, micro/nanopatterns spaced at large distances.

Key words

Microcontact printing Protein Poly(methyl methacrylate) Nanoimprint lithography 

Notes

Acknowledgments

E.M. is grateful to the Spanish Ministry of Science and Education for the provision of grants through the I3 system. We acknowledge financial support from the Spanish Ministerio de Ciencia e Innovación through the project entitled “Regenerative Stem Cell Therapies for Heart Failure” and from the Science Support Program of the Fundación Marcelino Botín.

References

  1. 1.
    Whitesides, G.M., Ostuni, E., Takayama, S., Jiang, X.Y., Ingber, D.E. (2001) Soft lithography in biology and biochemistry. Annu Rev Biomed Eng. 3, 335–373.CrossRefGoogle Scholar
  2. 2.
    Kane, R. S., Takayama, S., Ostuni, E., Ingber, D. E., Whitesides, G. M. (1999) Patterning proteins and cells using soft lithography. Biomaterials 20, 2363–2376.CrossRefGoogle Scholar
  3. 3.
    Xia, Y., Whitesides, G.M. (1998) Soft lithography. Angew Chem Int Ed 37, 550–575.CrossRefGoogle Scholar
  4. 4.
    Hui, C. Y., Jagota, A., Lin, Y. Y., Kramer, E. J. (2002) Constraints on microcontact printing imposed by stamp deformation. Langmuir 18, 1394–1407.CrossRefGoogle Scholar
  5. 5.
    Lauer, L., Klein C., Offenhäusser, A. (2001) Spot compliant neuronal networks by structure optimized micro-contact printing. Biomaterials 22, 1925–1932.CrossRefGoogle Scholar
  6. 6.
    Delamarche, E., Schmid, H., Biebuyck, H. A., Michel, B. (1997) Stability of molded polydimethylsiloxane microstructures. Adv. Mater. 9, 741–746.CrossRefGoogle Scholar
  7. 7.
    Bessueille, F., Pla-Roca, M., Mills, C. A., Martinez, E., Samitier, J., Errachid A. (2005) Submerged microcontact printing (SμCP): an unconventional printing technique of thiols using high aspect ratio, elastomeric stamps. Langmuir 21, 12060–12063.CrossRefGoogle Scholar
  8. 8.
    Renault, J.P., Bernard, A., Bietsch, A., Michel, B., Bosshard, H.R., Delamarche, E., Kreiter, M., Hecht, B., Wild, U.P. (2003) Fabricating arrays of single protein molecules on glass using microcontact printing, J. Phys. Chem. B 107, 703–711.CrossRefGoogle Scholar
  9. 9.
    Hong-Wei, L., Beinn, V. O., Muir, G. F., Huck, W. T. S. (2003) Nanocontact printing: a route to sub-50-nm-scale chemical and biological patterning. Langmuir 19, 1963–65.CrossRefGoogle Scholar
  10. 10.
    Pla-Roca, M., Fernandez, J. G., Mills, C. A., Martínez, E., Samitier J. (2007) Micro/nanopatterning of proteins via contact printing using high aspect ratio PMMA stamps and nanoimprint apparatus. Langmuir 23, 8614–8618.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Elena Martínez
    • 1
    • 2
    Email author
  • Mateu Pla-Roca
    • 3
  • Josep Samitier
    • 1
    • 4
    • 2
  1. 1.Nanobioengineering LaboratoryInstitute for Bioengineering of Catalonia (IBEC)BarcelonaSpain
  2. 2.Centro de Investigación Biomédica en Red en BioingenieríaBiomateriales y Nanomedicina (CIBER-BBN)BarcelonaSpain
  3. 3.Laboratory of Surface science and TechnologySwiss Federal Institute of Technology (ETH Zurich)ZurichSwitzerland
  4. 4.Department of ElectronicsUniversity of BarcelonaBarcelonaSpain

Personalised recommendations