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Protein Adsorption Characterization

  • M. Cristina L. MartinsEmail author
  • Susana R. Sousa
  • Joana C. Antunes
  • Mário A. Barbosa
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 811)

Abstract

Protein adsorption from (aqueous) solutions onto a (solid) surface is a common process that takes place at biological interfaces. This phenomenon, that spontaneously occurs, changes the properties of the surface and can induce structural modifications on proteins. Proteins in solution can be easily identified/quantified using classical biochemical methods. However, adsorbed proteins are more difficult to assess since they are always associated with a substrate. The selection of the analytical method depends on the type of substrate used, the amount of adsorbed protein, the type of solution (single protein solution vs. complex biological media), and the type of information that is demanded (quantification of the adsorbed protein, adsorption kinetics, conformation, and orientation of the adsorbed protein). Until now, none of the techniques available are capable by its own to characterize all the protein adsorption process. Therefore, a multitechnique analysis is required. During this chapter, the methodologies to measure human serum albumin to poly(ethylene terephthalate) using the three different techniques, radiolabeling, ellipsometry, and quartz crystal microbalance with dissipation – QCM-D, are described in detail. The specific preparation of polymeric surfaces to be used with each technique is also presented.

Key words

125I radiolabeling Iodogen method Protein adsorption Ellipsometry Quartz crystal microbalance Biomaterial Surface 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • M. Cristina L. Martins
    • 1
    Email author
  • Susana R. Sousa
    • 1
    • 2
  • Joana C. Antunes
    • 3
  • Mário A. Barbosa
    • 4
  1. 1.Divisão de Biomateriais, INEB – Instituto de Engenharia BiomédicaUniversidade do PortoPortoPortugal
  2. 2.Departamento de Engenharia QuímicaREQUIMTE/Instituto Superior de Engenharia do PortoPortoPortugal
  3. 3.Faculdade de Engenharia, Divisão de Biomateriais, INEB – Instituto de Engenharia BiomédicaUniversidade do PortoPortoPortugal
  4. 4.Faculdade de Engenharia, Divisão de Biomateriais, Departamento de Engenharia Metalúrgica e Materiais, INEB – Instituto de Engenharia BiomédicaUniversidade do PortoPortoPortugal

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