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Modulation of Cellular Respiration by Endogenously Produced Nitric Oxide in Rat Hippocampal Slices

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Mitochondrial Bioenergetics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 810))

Abstract

Nitric oxide (•NO) is a ubiquitous signaling molecule that participates in neuromolecular phenomena associated with memory formation as well as in excitotoxicity. In the hippocampus, neuronal •NO production is coupled to the activation of the NMDA-type of glutamate receptor. More recently, Cytochrome c oxidase has emerged as a novel target for •NO, which competes with O2 for binding to this mitochondrial complex. This reaction establishes •NO not only as a regulator of cellular metabolism but possibly also as a regulator of mitochondrial production of reactive oxygen species which participate in cellular signaling. A major gap in the understanding of •NO bioactivity, namely, in the hippocampus, has been the lack of knowledge of its concentration dynamics. Here, we present a detailed description of the simultaneous recording of •NO and O2 concentration dynamics in rat hippocampal slices. Carbon fiber microelectrodes are fabricated and applied for real-time measurements of both gases in a system close to in vivo models. This approach allows for a better understanding of the current paradigm by which an intricate interplay between •NO and O2 regulates cellular respiration.

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Acknowledgment

This work was funded by grant PTDC/SAU-NEU/108992/2008 from FCT (Portugal).

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Authors and Affiliations

  1. Center for Neuroscience and Cell Biology, University of Coimbra, R. Larga 6, Coimbra, 3004-504, Portugal

    Ana Ledo

  2. Faculty of Pharmacy, Center for Neurosciences and Cell Biology, University of Coimbra, R. Larga, 3004-504, 3030, Coimbra, Portugal

    R. M. Barbosa

  3. Faculty of Pharmacy, Center for Neurosciences and Cell Biology, University of Coimbra, Coimbra, Portugal

    J. Laranjinha

Authors
  1. Ana Ledo
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  2. R. M. Barbosa
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  3. J. Laranjinha
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Corresponding author

Correspondence to Ana Ledo .

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Editors and Affiliations

  1. Center for Neurosciences and Cell Biolog, Universidad de Coimbra, Lg. Marquês de Pombal 3046, Coimbra, 3004-517, Portugal

    Carlos M. Palmeira

  2. Centro de Neurociencias, Universidad de Coimbra, Lg. Marquês de Pombal 3046, Coimbra, 3004-517, Portugal

    António J. Moreno

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Ledo, A., Barbosa, R.M., Laranjinha, J. (2012). Modulation of Cellular Respiration by Endogenously Produced Nitric Oxide in Rat Hippocampal Slices. In: Palmeira, C., Moreno, A. (eds) Mitochondrial Bioenergetics. Methods in Molecular Biology, vol 810. Humana Press. https://doi.org/10.1007/978-1-61779-382-0_5

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  • DOI: https://doi.org/10.1007/978-1-61779-382-0_5

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-381-3

  • Online ISBN: 978-1-61779-382-0

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