Abstract
The cDNA display method is a robust in vitro display technology that converts an unstable mRNA–protein fusion (mRNA display) to a stable mRNA/cDNA–protein fusion (cDNA display) whose cDNA is covalently linked to its encoded protein using a well-designed puromycin linker. We provide technical details for preparing cDNA display molecules and for the synthesis of the puromycin linker for the purpose of screening the functional proteins and peptides.
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Acknowledgments
Much of the contents of this work comprises the collected wisdom of a number of colleagues. The authors would like to thank Prof. Yuzuru Husimi for providing helpful comments to the manuscript.
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Ueno, S., Nemoto, N. (2012). cDNA Display: Rapid Stabilization of mRNA Display. In: Douthwaite, J., Jackson, R. (eds) Ribosome Display and Related Technologies. Methods in Molecular Biology, vol 805. Springer, New York, NY. https://doi.org/10.1007/978-1-61779-379-0_8
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DOI: https://doi.org/10.1007/978-1-61779-379-0_8
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