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Ribosome Display and Related Technologies pp 191–212Cite as

Evolution of Protein Stability Using Ribosome Display

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 805))

Abstract

The opportunity to enhance protein stability has a number of potential benefits for biological therapeutics – for example extending in vivo half-life, enabling a longer shelf life, reducing the propensity to aggregate, or enabling soluble expression. Engineering protein stability has been attempted empirically, rationally, and using directed evolution based on phage display. Ribosome display is a powerful in vitro technology for the selection and directed evolution of proteins. Ribosome display is typically used for the generation of high-affinity proteins and peptides. This method extends the utility of ribosome display to selecting for stability, defined as the propensity of a molecule to exist in its folded and active state.

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Author information

Authors and Affiliations

  1. Department of Antibody Discovery and Protein Engineering, MedImmune Limited, Cambridge, UK

    Andrew Buchanan

Authors
  1. Andrew Buchanan
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Corresponding author

Correspondence to Andrew Buchanan .

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Editors and Affiliations

  1. , Antibody Discovery & Protein Engineering, MedImmune Limited, Granta Park, Cambridge, CB21 6GH, United Kingdom

    Julie A. Douthwaite

  2. , Antibody Discovery & Protein Engineering, MedImmune Limited, Granta Park, Cambridge, CB21 6GH, United Kingdom

    Ronald H. Jackson

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© 2012 Springer Science+Business Media, LLC

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Buchanan, A. (2012). Evolution of Protein Stability Using Ribosome Display. In: Douthwaite, J., Jackson, R. (eds) Ribosome Display and Related Technologies. Methods in Molecular Biology, vol 805. Springer, New York, NY. https://doi.org/10.1007/978-1-61779-379-0_11

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  • DOI: https://doi.org/10.1007/978-1-61779-379-0_11

  • Published:

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-61779-378-3

  • Online ISBN: 978-1-61779-379-0

  • eBook Packages: Springer Protocols

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