Abstract
We present here a method for construction of carbohydrate cluster microarrays. This technology utilizes a 3-dimensional poly(amidoamine) starburst dendrimer monolayer assembled on glass surface, which is functionalized with terminal hydrazide groups for site-specific coupling of carbohydrates without prior chemical derivatization. Microwave radiation energy is applied to accelerate carbohydrate coupling on chips. Since this bioarray platform is designed to present sugar chains in defined orientation and cluster configurations, it is particularly suitable for exploration of the structural and conformational diversities of glycoepitopes and their functional properties.
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Acknowledgments
This work was supported by Grant Number 1R43 RR023763-01 and 2R44 RR023763-02 (X.C. Zhou) from the National Center for Research Resources Services (NCRR) and U01CA128416 (D. Wang) from National Cancer Institute (NCI), components of the National Institutes of Health (NIH). The content is solely the responsibility of the authors and does not necessarily represent the official views of the NCRR or NCI or the National Institutes of Health.
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Zhou, X., Zhang, J., Wang, D. (2012). Microwave-Assisted Method for Fabrication of Carbohydrate Cluster Microarrays on 3-Dimensional Hydrazide–Dendrimer Substrate. In: Chevolot, Y. (eds) Carbohydrate Microarrays. Methods in Molecular Biology, vol 808. Humana Press. https://doi.org/10.1007/978-1-61779-373-8_27
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DOI: https://doi.org/10.1007/978-1-61779-373-8_27
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