Abstract
The parathyroid cells are highly differentiated with more or less their only function to secrete parathyroid hormone in response to the extracellular calcium level. Tumours from the parathyroid glands are >99% benign, and have a slow proliferation rate. Culture of parathyroid cells is known to be very difficult most likely due to the high differentiation level. This chapter reveals some details in order how to get parathyroid cells to survive in culture after dispersion of normal bovine parathyroid glands or pathological human parathyroid tumours. Detailed protocols describing cell dispersion with collagenase, short-term cultures, and establishment of long-term cultures are presented.
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References
Grimelius L, Åkerström G, Johansson H, Juhlin C, Rastad J 1991 The parathyroid glands. In: Kovacs K, Asa L eds. Functional endocrine pathology. Boston: Blackwell Scientific Publishers; 375–395.
Liu W, Hellman P, Li Q, Yu W-R, Juhlin C, Nordlinder H, Rollman O, Åkerström G, Törmä H, Melhus H 1996 Biosynthesis and function of all-trans- and 9-cis-retinoic acid in parathyroid cells. Biochem Biophys Res Commun 229:922–929.
Ridefelt P, Nygren P, Hellman P, Larsson R, Rastad J, Åkerström G, Gylfe E 1992 Regulation of parathyroid hormone release in normal and pathological parathyroid cells exposed to modulators of protein kinase C. Acta Endocrinol 126:505–509.
Brown E 1991 Extracellular Ca2+ sensing, regulation of parathyroid cell function, and role of Ca2+ and other ions as extracellular (first) messengers. Physiol Rev 71:371–411.
Rudberg C, Grimelius L, Johansson H, Ljunghall S, Odselius R, Pertoft H, Wide L, Åkerström G 1986 Alterations in density, morphology and parathyroid hormone release of dispersed parathyroid cells from patients with hyperparathyroidism. Acta Path Microbiol Immunol Scand Sect A 94:253–261.
Liu W, Ridefelt P, Akerstrom G, Hellman P 2001 Differentiation of human parathyroid cells in culture. J Endocrinol 168:417–425.
Bjorklund P, Akerstrom G, Westin G 2007 Activated beta-catenin in the novel human parathyroid tumor cell line sHPT-1. Biochem Biophys Res Commun 352:532–536.
Roussanne M, Gogusev J, Hory B, Duchambon P, Souberbielle J, Nabarra B, Pierrat D, Sarfati E, Drueke T, Bourdeau A 1998 Persistance of Ca2 + −sensing receptor expression in functionally active, long-term human parathyroid cultures. J Bone Min Res 13:354–362.
Roussanne MC, Lieberherr M, Souberbielle JC, Sarfati E, Drueke T, Bourdeau A 2001 Human parathyroid cell proliferation in res-ponse to calcium, NPS R-467, calcitriol and phosphate. Eur J Clin Invest 31:610–616.
Brandi ML, Fitzpatrick LA, Coon HG, Aurbach GD 1986 Bovine parathyroid cells: Cultures maintained for more than 140 populations doublings. Proc Natl Acad Sci USA 83:1709–1713.
Ridefelt P, Hellman P, Wallfelt C, Åkerström G, Rastad J, Gylfe E 1992 Neomycin interacts with Ca2+ sensing of normal and adenomatous parathyroid cells. Mol Cell Endocrinol 83:211–218.
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Björklund, P., Hellman, P. (2012). Culture of Parathyroid Cells. In: Mitry, R., Hughes, R. (eds) Human Cell Culture Protocols. Methods in Molecular Biology, vol 806. Humana Press. https://doi.org/10.1007/978-1-61779-367-7_4
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DOI: https://doi.org/10.1007/978-1-61779-367-7_4
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