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Laser Microdissection Microscopy: Application to Cell Culture

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Book cover Human Cell Culture Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 806))

Abstract

Laser microdissection (LMD) microscopy allows isolation of specific cell populations to target their ­molecular profile. There are several different types of LMD microscopes, but they are all based on the same principle. A laser beam is used to cut out cells or tissues of interest from a histological section, cytology preparations, or live cells from tissue cultures. Live cells can be isolated using LMD and processed for downstream molecular work. RNA, DNA, and protein isolation is possible from a small number of cells and the material is suitable for further real-time PCR, ELISA, Western Blotting, and protein microarray analysis.

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References

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Author information

Authors and Affiliations

  1. Institute of Liver Studies, King’s College London School of Medicine, London, UK

    Ahlam Mustafa, Cathy Cenayko, Ragai R. Mitry & Alberto Quaglia

Authors
  1. Ahlam Mustafa
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  2. Cathy Cenayko
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  3. Ragai R. Mitry
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  4. Alberto Quaglia
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Corresponding author

Correspondence to Ahlam Mustafa .

Editor information

Editors and Affiliations

  1. Institute of Liver Studies, King’s College Hospital, Denmark Hill, London, SE5 9RS, United Kingdom

    Ragai R. Mitry

  2. School of Medicine, Inst. Liver Studies, King's College London, Bessemer Rd., London, SE5 9RS, United Kingdom

    Robin D. Hughes

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© 2012 Springer Science+Business Media, LLC

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Mustafa, A., Cenayko, C., Mitry, R.R., Quaglia, A. (2012). Laser Microdissection Microscopy: Application to Cell Culture. In: Mitry, R., Hughes, R. (eds) Human Cell Culture Protocols. Methods in Molecular Biology, vol 806. Humana Press. https://doi.org/10.1007/978-1-61779-367-7_25

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  • DOI: https://doi.org/10.1007/978-1-61779-367-7_25

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-366-0

  • Online ISBN: 978-1-61779-367-7

  • eBook Packages: Springer Protocols

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