Abstract
Genome-wide association studies and genetic linkage studies have created a growing list of proteins related to disease. Small molecules can serve as useful probes of function for these proteins in a cellular setting or may serve as leads for therapeutic development. High-throughput and general binding assays may provide a path for discovering small molecules that target proteins for which little is known about structure or function or for which conventional functional assays have failed. One such binding assay involves small-molecule microarrays (SMMs) containing compounds that have been arrayed and immobilized onto a solid support. The SMMs can be incubated with a protein target of interest and protein–small molecule interactions may be detected using a variety of fluorescent readouts. Several suitable methods for manufacturing SMMs exist and different immobilization methods may be more or less preferable for any given application. Here, we describe protocols for covalent capture of small molecules using an isocyanate-coated glass surface and detection of binding using purified protein.
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Acknowledgments
The authors would like to thank Michelle Palmer, Yan-Ling Zhang, Gil Walzer, Hong Chen, Jacob Asiedu, Lisa Marcaurelle, Michael Foley, James Bradner, Olivia McPherson, and Stuart Schreiber for materials, technical support, or advice that was relevant to developing this updated protocol. Work described herein was funded with Federal funds from the National Cancer Institute’s Initiative for Chemical Genetics, National Institutes of Health, under contract no. N01-CO-12400.
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Casalena, D.E., Wassaf, D., Koehler, A.N. (2012). Ligand Discovery Using Small-Molecule Microarrays. In: Drewes, G., Bantscheff, M. (eds) Chemical Proteomics. Methods in Molecular Biology, vol 803. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-364-6_17
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DOI: https://doi.org/10.1007/978-1-61779-364-6_17
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