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Protein Expression in Mammalian Cells pp 125–135Cite as

Post-transcriptional Regulatory Elements for Enhancing Transient Gene Expression Levels in Mammalian Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 801))

Abstract

Low yield from transient gene expression in mammalian cells limits its application to areas where large amount of proteins are needed. One effective approach to enhance transient gene expression levels is to use post-transcriptional regulatory elements (PTREs). We have evaluated the effect of five PTREs on the transient gene expression of three proteins in two cell lines. Most of the elements increased expression but exhibited cell-specific and gene-specific effects. The tripartite leader sequence of human adenovirus mRNA linked with a major late promoter enhancer gave the most universal and highest enhancement of gene expression levels. It increased the expression of all three proteins in HEK293 cells and two proteins in CHO K1 cells by 3.6- to 7.6-fold. Combinations of multiple PTREs increased protein expression as much as 10.5-fold.

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Acknowledgments

This work was supported by the Biomedical Research Council/Science and Engineering Research Council of A*STAR (Agency for Science, Technology and Research), Singapore.

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Authors and Affiliations

  1. Agency for Science, Technology and Research, Bioprocessing Technology Institute, Singapore, Singapore

    Mariati, Steven C. L. Ho, Miranda G. S. Yap & Yuansheng Yang

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  2. Steven C. L. Ho
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  3. Miranda G. S. Yap
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  4. Yuansheng Yang
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Corresponding author

Correspondence to Yuansheng Yang .

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Editors and Affiliations

  1. SAIC-Frederick, Inc., Protein Expression Laboratory, National Cancer Institute, Frederick, Boyles Street 1050, Frederick, 21702, Maryland, USA

    James L Hartley

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Mariati, Ho, S.C.L., Yap, M.G.S., Yang, Y. (2012). Post-transcriptional Regulatory Elements for Enhancing Transient Gene Expression Levels in Mammalian Cells. In: Hartley, J. (eds) Protein Expression in Mammalian Cells. Methods in Molecular Biology, vol 801. Humana Press. https://doi.org/10.1007/978-1-61779-352-3_9

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  • DOI: https://doi.org/10.1007/978-1-61779-352-3_9

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-351-6

  • Online ISBN: 978-1-61779-352-3

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