Strategies for Efficient Transfection of CHO-Cells with Plasmid DNA

  • Renate KunertEmail author
  • Karola Vorauer-Uhl
Part of the Methods in Molecular Biology book series (MIMB, volume 801)


Stable cell lines of Chinese hamster ovary (CHO) cells are the predominant source of commercial ­biopharmaceutical proteins. Because making suitable CHO cell lines is time-consuming and costly, ­preliminary experiments with transient expression are usually performed to optimize as many protein ­production parameters as possible. Here, we describe protocols for optimizing expression in transient expression experiments and isolating stable CHO cell lines using two types of self-made reagents, namely, lipoplexes and polyplexes.

Key words

Transfection Chinese hamster ovary Transient Stable Lipoplex Polyplex 



We thank Hannes Reisinger for technical help and Marion Tschernutter for her help with manuscript preparation.


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of BiotechnologyUniversity of Natural Resources and Life SciencesViennaAustria

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