Abstract
Small molecule-immobilized affinity matrices are indispensable tools in chemical genomics to screen and purify protein targets for biologically active small molecules. Usually, prior to immobilization, small molecules have to be derivatized at a position that does not significantly abrogate the intrinsic biological activity, or chemically synthesized to have an appropriate functional group for the immobilization chemistry. Here, we describe a photo-cross-linking technique to immobilize biologically active small molecules for protein target screening, without the need for chemical derivatization.
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Acknowledgments
This work is supported by a Grant-in-Aid for Young Scientists (A) (No. 19681023; to NK), a Grant-in-Aid for Scientific Research (B) (No. 22310132; to NK), and a Grant-in-Aid for JAPS fellows (No. 2255053; to HT). We also thank Prof. Takayuki Doi and Dr. Masahito Yoshida for their assistance with the LC/MS analysis.
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Takayama, H., Moriya, T., Kanoh, N. (2012). Preparation of Photo-Cross-Linked Small Molecule Affinity Matrices for Affinity Selection of Protein Targets for Biologically Active Small Molecules. In: Zanders, E. (eds) Chemical Genomics and Proteomics. Methods in Molecular Biology, vol 800. Humana Press. https://doi.org/10.1007/978-1-61779-349-3_6
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DOI: https://doi.org/10.1007/978-1-61779-349-3_6
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