Skip to main content
SpringerLink
Log in

Analysis of the Immune Response to Neisseria meningitidis Using a Proteomics Approach

  • Protocol
  • First Online:
Book cover Neisseria meningitidis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 799))

Abstract

The availability of Neisseria genome sequences together with improvements in proteomic technologies provide the opportunity to study at high resolution the immune response to Neisseria meningitidis. In this chapter, we describe a protocol that combines two-dimensional (2D) SDS-PAGE of meningococcal outer membranes with western blotting of human antisera to identify proteins associated with the development of protective antibody responses. This methodology can identify putative vaccine candidates for incorporation in a multi-component serogroup B meningococcal vaccine.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 159.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Beveridge TJ (1999) Structures of gram-negative cell walls and their derived membrane vesicles. J Bacteriol 181: 4725–4733.

    Article  CAS  Google Scholar 

  2. Tettelin H, Saunders NJ, Heidelberg J et al (2000) Complete genome sequence of Neisseria meningitidis serogroup B strain MC58. Science 287: 1809–1815.

    Article  CAS  Google Scholar 

  3. Parkhill J, Achtman M, James KD et al (2000) Complete DNA sequence of a serogroup A strain of Neisseria meningitidis Z2491. Nature 404: 502–506.

    Article  CAS  Google Scholar 

  4. Bentley SD, Vernikos GS, Snyder LA et al (2007) Meningococcal genetic variation mechanisms viewed through comparative analysis of serogroup C strain FAM18. PLoS Genet 3: e23.

    Article  Google Scholar 

  5. Hotopp JC, Grifantini R, Kumar N et al (2006) Comparative genomics of Neisseria meningitidis: core genome, islands of horizontal transfer and pathogen-specific genes. Microbiology 152: 3733–3749.

    Article  Google Scholar 

  6. Rabilloud T (2009) Membrane proteins and proteomics: love is possible, but so difficult. Electrophoresis 30 Suppl 1: S174–S180.

    Article  Google Scholar 

  7. Williams JN, Skipp PJ, Humphries HE et al (2007) Proteomic analysis of outer membranes and vesicles from wild-type serogroup B Neisseria meningitidis and a lipopolysaccharide-deficient mutant. Infect Immun 75: 1364–1372.

    Article  CAS  Google Scholar 

  8. Vaughan TE, Skipp PJ, O’Connor CD et al (2006) Proteomic analysis of Neisseria lactamica and Neisseria meningitidis outer membrane vesicle vaccine antigens. Vaccine 24: 5277–5293.

    Article  CAS  Google Scholar 

  9. Vipond C, Wheeler JX, Jones C et al (2005) Characterization of the protein content of a meningococcal outer membrane vesicle vaccine by polyacrylamide gel electrophoresis and mass spectrometry. Hum Vaccin 1: 80–84.

    Article  CAS  Google Scholar 

  10. Uli L, Castellanos-Serra L, Betancourt L et al (2006) Outer membrane vesicles of the VA-MENGOC-BC vaccine against serogroup B of Neisseria meningitidis: Analysis of protein components by two-dimensional gel electrophoresis and mass spectrometry. Proteomics 6: 3389–3399.

    Article  CAS  Google Scholar 

  11. Ferrari G, Garaguso I, Adu-Bobie J et al (2006) Outer membrane vesicles from group B Neisseria meningitidis gna33 mutant: Proteomic and immunological comparison with detergent-derived outer membrane vesicles. Proteomics 6: 1856–1866.

    Article  CAS  Google Scholar 

  12. Vipond C, Suker J, Jones C et al (2006) Proteomic analysis of a meningococcal outer membrane vesicle vaccine prepared from the group B strain NZ98/254. Proteomics 6: 4203.

    Article  CAS  Google Scholar 

  13. Williams JN, Skipp PJ, O’Connor CD et al (2009) Immunoproteomic Analysis of the Development of Natural Immunity in Subjects Colonized by Neisseria meningitidis Reveals Potential Vaccine Candidates. Infect Immun 77: 5080–5089.

    Article  CAS  Google Scholar 

  14. Mendum TA, Newcombe J, McNeilly CL et al (2009) Towards the Immunoproteome of Neisseria meningitidis. PLoS ONE 4: e5940.

    Article  Google Scholar 

  15. Abel A, Sanchez S, Arenas J et al (2007) Bioinformatic analysis of outer membrane proteome of Neisseria meningitidis and Neisseria lactamica. Int Microbiol 10: 5–11.

    CAS  PubMed  Google Scholar 

  16. Bernardini G, Renzone G, Comanducci M et al (2004) Proteome analysis of Neisseria meningitidis serogroup A. Proteomics 4: 2893–2926.

    Article  CAS  Google Scholar 

  17. Pennington K, McGregor E, Beasley CL et al (2004) Optimization of the first dimension for separation by two-dimensional gel electrophoresis of basic proteins from human brain tissue. Proteomics 4: 27–30.

    Article  CAS  Google Scholar 

  18. Anonymous, 2-D Electrophoresis: Principles and Methods, in: Anonymous, Handbook 80-6429-60AC,GE Healthcare, 2004, pp. 1–168.

    Google Scholar 

  19. Jones GR, Williams JN, Christodoulides M et al (2000) Lack of immunity in university students prior to an outbreak of serogroup C meningococcal infection. J Infect Dis 181: 1172–1175.

    Article  CAS  Google Scholar 

  20. Jones GR, Christodoulides M, Brooks JL et al (1998) Dynamics of carriage of Neisseria meningitidis in a group of military recruits: subtype stability and specificity of the immune response following colonisation. J Infect Dis 178: 451–459.

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by Meningitis UK and Wessex Medical Research.

Author information

Authors and Affiliations

  1. Division of Infection, Inflammation, and Immunity, Sir Henry Wellcome Laboratories, University of Southampton Medical School, Southampton, UK

    Jeannette N. Williams, Myron Christodoulides & John E. Heckels

Authors
  1. Jeannette N. Williams
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Myron Christodoulides
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. John E. Heckels
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jeannette N. Williams .

Editor information

Editors and Affiliations

  1. Medical School, Molecular Microbiology and Infection, University of Southampton, Tremona Road, Southampton, SO16 6YD, Hampshire, United Kingdom

    Myron Christodoulides

Rights and permissions

Reprints and permissions

Copyright information

© 2012 Springer Science+Business Media, LLC

About this protocol

Cite this protocol

Williams, J.N., Christodoulides, M., Heckels, J.E. (2012). Analysis of the Immune Response to Neisseria meningitidis Using a Proteomics Approach. In: Christodoulides, M. (eds) Neisseria meningitidis. Methods in Molecular Biology, vol 799. Humana, Totowa, NJ. https://doi.org/10.1007/978-1-61779-346-2_20

Download citation

  • DOI: https://doi.org/10.1007/978-1-61779-346-2_20

  • Published:

  • Publisher Name: Humana, Totowa, NJ

  • Print ISBN: 978-1-61779-345-5

  • Online ISBN: 978-1-61779-346-2

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation