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Neisseria meningitidis pp 169–184Cite as

Methods for Studying Neisseria meningitidisBiofilms

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 799))

Abstract

Neisseria meningitidisis an organism whose environmental niche is limited to the human host. It can frequently colonize the human nasopharynx and has the ability to cause severe systemic infections. These infections can be sporadic, endemic or occur in outbreaks associated with more virulent meningococcal strains. Studies have demonstrated that the meningococcus can form biofilms both in vivo and ex vivo. In this chapter, we discuss methods to establish biofilms in the laboratory for in-depth biochemical, genetic, or microscopic studies.

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Acknowledgements

These methods were developed with the support of US Public Health Service NIAID grant AI045728.

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Authors and Affiliations

  1. The Department of Microbiology, Carver College of Medicine, The University of Iowa, Iowa City, IA, USA

    Michael A. Apicella, Jianqiang Shao & R. Brock Neil

Authors
  1. Michael A. Apicella
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  2. Jianqiang Shao
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  3. R. Brock Neil
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Corresponding author

Correspondence to Michael A. Apicella .

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Editors and Affiliations

  1. Medical School, Molecular Microbiology and Infection, University of Southampton, Tremona Road, Southampton, SO16 6YD, Hampshire, United Kingdom

    Myron Christodoulides

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Apicella, M.A., Shao, J., Neil, R.B. (2012). Methods for Studying Neisseria meningitidisBiofilms. In: Christodoulides, M. (eds) Neisseria meningitidis. Methods in Molecular Biology, vol 799. Humana, Totowa, NJ. https://doi.org/10.1007/978-1-61779-346-2_11

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  • DOI: https://doi.org/10.1007/978-1-61779-346-2_11

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  • Publisher Name: Humana, Totowa, NJ

  • Print ISBN: 978-1-61779-345-5

  • Online ISBN: 978-1-61779-346-2

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