Abstract
Resistance loading provides an important tool for understanding skeletal muscle responses and adaptations to various perturbations. A model using anesthetized rodents provides the means to control the input parameters carefully, and to measure the output parameters of each muscle contraction. Unilateral models of anesthetized loading also provide the advantage of comparing an unloaded and loaded muscle from the same animal. Voluntary models for resistance loading arguably provide a more “physiological response” but it also introduces more variability in the input parameters, which can be affected by the stimulus used to motivate the animal to exercise. After either acute or chronic periods of muscle loading, the loaded muscles can be removed and various signaling proteins can be determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) or enzyme assays. Several assays are described, which provide an indication of downstream markers for oxidative stress.
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Ryan, M. J., Dudash, H. J., Docherty, M., Geronilla, K. B., Baker, B. A., Haff, G. G., Cutlip, R. G., and Alway, S. E. (2008) Aging-Dependent Regulation of Antioxidant Enzymes and Redox Status in Chronically Loaded Rat Dorsiflexor Muscles, J Gerontol A Biol Sci Med Sci 63, 1015–1026.
McBride, J. M., Kraemer, W. J., Triplett-McBride, T., and Sebastianelli, W. (1998) Effect of resistance exercise on free radical production, Med. Sci. Sports Exerc. 30, 67–72.
Gianni, P., Jan, K. J., Douglas, M. J., Stuart, P. M., and Tarnopolsky, M. A. (2004) Oxidative stress and the mitochondrial theory of aging in human skeletal muscle, Exp. Gerontol. 39, 1391–1400.
Baker, B. A., Hollander, M. S., Kashon, M. L., and Cutlip, R. G. (2010) Effects of glutathione depletion and age on skeletal muscle performance and morphology following chronic stretch-shortening contraction exposure, Eur. J. Appl. Physiol. 108, 619–630.
Ryan, M. J., Jackson, J. R., and Alway, S. E. (2010) Suppression of oxidative stress by resveratrol after isometric contractions in gastrocnemius muscles of aged mice, J Gerontol A Biol Sci Med Sci 65, 815–831.
Harman D. (1956) Aging: a theory based on free radical and radiation chemistry, J Gerontol. 11, 298–300.
Uchiyama, S., Tsukamoto, H., Yoshimura, S., and Tamaki, T. (2006) Relationship between oxidative stress in muscle tissue and weight-lifting-induced muscle damage, Pflugers Archiv-European Journal of Physiology 452, 109–116.
Urso, M. L. and Clarkson, P. M. (2003) Oxidative stress, exercise, and antioxidant supplementation, Toxicology 189, 41–54.
Baker, B. A., Hollander, M. S., Mercer, R. R., Kashon, M. L., and Cutlip, R. G. (2008) Adaptive stretch-shortening contractions: diminished regenerative capacity with aging, Appl Physiol Nutr Metab 33, 1181–1191.
Cutlip, R. G., Baker, B. A., Geronilla, K. B., Mercer, R. R., Kashon, M. L., Miller, G. R., Murlasits, Z., and Alway, S. E. (2006) Chronic exposure to stretch-shortening contractions results in skeletal muscle adaptation in young rats and maladaptation in old rats, Appl Physiol Nutr Metab 31, 573–587.
Geronilla, K. B., Miller, G. R., Mowrey, K. F., Wu, J. Z., Kashon, M. L., Brumbaugh, K., Reynolds, J., Hubbs, A., and Cutlip, R. G. (2003) Dynamic force responses of skeletal muscle during stretch-shortening cycles, Eur J Appl Physiol 90, 144–153.
Cutlip, R. G., Baker, B. A., Hollander, M., and Ensey, J. (2009) Injury and adaptive mechanisms in skeletal muscle, J Electromyogr Kinesiol 19, 358–372.
Cutlip, R. G., Geronilla, K. B., Baker, B. A., Kashon, M. L., Miller, G. R., and Schopper, A. W. (2004) Impact of muscle length during stretch-shortening contractions on real-time and temporal muscle performance measures in rats in vivo, J Appl Physiol 96, 507–516.
Gosselin, L. E. and Burton, H. (2002) Impact of initial muscle length on force deficit following lengthening contractions in mammalian skeletal muscle, Muscle Nerve 25, 822–827.
Cutlip, R. G., Baker, B. A., Geronilla, K. B., Kashon, M. L., and Wu, J. Z. (2007) The influence of velocity of stretch-shortening contractions on muscle performance during chronic exposure: age effects, Appl Physiol Nutr Metab 32, 443–453.
Baker, B. A., Mercer, R. R., Geronilla, K. B., Kashon, M. L., Miller, G. R., and Cutlip, R. G. (2007) Impact of repetition number on muscle performance and histological response, Med Sci Sports Exerc 39, 1275–1281.
Cutlip, R. G., Geronilla, K. B., Baker, B. A., Chetlin, R. D., Hover, I., Kashon, M. L., and Wu, J. Z. (2005) Impact of stretch-shortening cycle rest interval on in vivo muscle performance, Med Sci Sports Exerc 37, 1345–1355.
McCully, K. K. (1986) Exercise-induced injury to skeletal muscle, Fed. Proc 45, 2933–2936.
Lieber, R. L., Woodburn, T. M., and Friden, J. (1991) Muscle damage induced by eccentric contractions of 25% strain, J Appl Physiol 70, 2498–2507.
Brooks, S. V. and Faulkner, J. A. (1990) Contraction-induced injury: recovery of skeletal muscles in young and old mice, Am J Physiol 258, C436–C442.
Koh, T. J. and Brooks, S. V. (2001) Lengthening contractions are not required to induce protection from contraction-induced muscle injury, Am J Physiol Regul Integr Comp Physiol 281, R155–R161.
Figueiredo, P. A., Powers, S. K., Ferreira, R. M., Appell, H. J., and Duarte, J. A. (2009) Aging impairs skeletal muscle mitochondrial bioenergetic function, J Gerontol A Biol Sci Med Sci 64, 21–33.
Ji, L. L., Leeuwenburgh, C., Leichtweis, S., Gore, M., Fiebig, R., Hollander, J., and Bejma, J. (1998) Oxidative stress and aging. Role of exercise and its influences on antioxidant systems, Ann. N. Y. Acad. Sci. 854, 102–117.
Fulle, S., Protasi, F., Di Tano, G., Pietrangelo, T., Beltramin, A., Boncompagni, S., Vecchiet, L., and Fano, G. (2004) The contribution of reactive oxygen species to sarcopenia and muscle ageing, Exp Gerontol 39, 17–24.
McArdle, A. and Jackson, M. J. (2000) Exercise, oxidative stress and ageing, J Anat. 197 Pt 4, 539–541.
Reid, M. B. (2001) Invited Review: redox modulation of skeletal muscle contraction: what we know and what we don’t, J Appl Physiol 90, 724–731.
Bejma, J. and Ji, L. L. (1999) Aging and acute exercise enhance free radical generation in rat skeletal muscle, J Appl Physiol 87, 465–470.
Houston, M., Estevez, A., Chumley, P., Aslan, M., Marklund, S., Parks, D. A., and Freeman, B. A. (1999) Binding of xanthine oxidase to vascular endothelium. Kinetic characterization and oxidative impairment of nitric oxide-dependent signaling, J Biol Chem 274, 4985–4994.
Hellsten, Y., Hansson, H. A., Johnson, L., Frandsen, U., and Sjodin, B. (1996) Increased expression of xanthine oxidase and insulin-like growth factor I (IGF-I) immunoreactivity in skeletal muscle after strenuous exercise in humans, Acta Physiol Scand 157, 191–197.
Vina, J., Gimeno, A., Sastre, J., Desco, C., Asensi, M., Pallardo, F. V., Cuesta, A., Ferrero, J. A., Terada, L. S., and Repine, J. E. (2000) Mechanism of free radical production in exhaustive exercise in humans and rats; role of xanthine oxidase and protection by allopurinol, IUBMB Life 49, 539–544.
Sachdev, S. and Davies, K. J. A. (2008) Production, detection, and adaptive responses to free radicals in exercise, Free Radic Biol Med 44, 215–223.
Kryger, A. I. and Andersen, J. L. (2007) Resistance training in the oldest old: consequences for muscle strength, fiber types, fiber size, and MHC isoforms, Scand J Med. Sci. Sports 17, 422–430.
Roman, W. J., Fleckenstein, J., Straygundersen, J., Alway, S. E., Peshock, R., and Gonyea, W. J. (1993) Adaptations in the Elbow Flexors of Elderly Males After Heavy-Resistance Training, J Appl Physiol 74, 750–754.
Muller, F. L., Song, W., Liu, Y. H., Chaudhuri, A., Pieke-Dahl, S., Strong, R., Huang, T. T., Epstein, C. J., Roberts, L. J., Csete, M., Faulkner, J. A., and Van Remmen, H. (2006) Absence of CuZn superoxide dismutase leads to elevated oxidative stress and acceleration of age-dependent skeletal muscle atrophy, Free Radic. Biol Med. 40, 1993–2004.
Cutlip, R. G., Stauber, W. T., Willison, R. H., McIntosh, T. A., and Means, K. H. (1997) Dynamometer for rat plantar flexor muscles in vivo, Med. Biol Eng Comput. 35, 540–543.
Murlasits, Z., Cutlip, R. G., Geronilla, K. B., Rao, K. M., Wonderlin, W. F., and Alway, S. E. (2006) Resistance training increases heat shock protein levels in skeletal muscle of young and old rats, Exp Gerontol 41, 398–406.
Baker, B. A., Rao, K. M., Mercer, R. R., Geronilla, K. B., Kashon, M. L., Miller, G. R., and Cutlip, R. G. (2006) Quantitative histology and MGF gene expression in rats following SSC exercise in vivo, Med Sci Sports Exerc 38, 463–471.
Warren, G. L., Lowe, D. A., and Armstrong, R. B. (1999) Measurement tools used in the study of eccentric contraction-induced injury, Sports Med 27, 43–59.
Warren, G. L., Hayes, D. A., Lowe, D. A., Williams, J. H., and Armstrong, R. B. (1994) Eccentric contraction-induced injury in normal and hindlimb-suspended mouse soleus and EDL muscles, J Appl Physiol 77, 1421–1430.
Warren, G. L., Lowe, D. A., Hayes, D. A., Karwoski, C. J., Prior, B. M., and Armstrong, R. B. (1993) Excitation failure in eccentric contraction-induced injury of mouse soleus muscle, J Physiol 468, 487–499.
Ryan, M. J., Dudash, H. J., Docherty, M., Geronilla, K. B., Baker, B. A., Haff, G. G., Cutlip, R. G., and Alway, S. E. (2010) Vitamin E and C supplementation reduces oxidative stress, improves antioxidant enzymes, and postive muscle work in chronically loaded muscles of aged rats, Exp. Gerontol. 45(11), 882–895.
Davis, J., Kaufman, K. R., and Lieber, R. L. (2003) Correlation between active and passive isometric force and intramuscular pressure in the isolated rabbit tibialis anterior muscle, J Biomech 36, 505–512.
Willems, M. E. and Stauber, W. T. (1999) Isometric and concentric performance of electrically stimulated ankle plantar flexor muscles in intact rat, Exp Physiol 84, 379–389.
Baker, B. A. and Cutlip, R. G. (2010) Skeletal muscle injury versus adaptation with aging: novel insights on perplexing paradigms, Exerc Sport Sci Rev 38, 10–16.
Tietze, F. (1969) Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: applications to mammalian blood and other tissues, Anal. Biochem 27, 502–522.
Afzal, M., Afzal, A., Jones, A., and Armstrong, D. (2002) A rapid method for the quantification of GSH and GSSG in biological samples, Methods Mol Biol 186, 117–122.
Zhou, M., Diwu, Z., Panchuk-Voloshina, N., and Haugland, R. P. (1997) A stable nonfluorescent derivative of resorufin for the fluorometric determination of trace hydrogen peroxide: applications in detecting the activity of phagocyte NADPH oxidase and other oxidases, Anal. Biochem 253, 162–168.
Yin, B., Whyatt, R. M., Perera, F. P., Randall, M. C., Cooper, T. B., and Santella, R. M. (1995) Determination of 8-hydroxydeoxyguanosine by an immunoaffinity chromatography-monoclonal antibody-based ELISA, Free Radic Biol Med 18, 1023–1032.
Chiou, C. C., Chang, P. Y., Chan, E. C., Wu, T. L., Tsao, K. C., and Wu, J. T. (2003) Urinary 8-hydroxydeoxyguanosine and its analogs as DNA marker of oxidative stress: development of an ELISA and measurement in both bladder and prostate cancers, Clin Chim. Acta 334, 87–94.
Senapathy, P., Ali, M. A., and Jacob, M. T. (1985) Mechanism of coupling periodate-oxidized nucleosides to proteins, FEBS Lett 190, 337–341.
Esterbauer, H., Schaur, R. J., and Zollner, H. (1991) Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes, Free Radic Biol Med 11, 81–128.
Gerard-Monnier, D., Erdelmeier, I., Regnard, K., Moze-Henry, N., Yadan, J. C., and Chaudiere, J. (1998) Reactions of 1-methyl-2-phenylindole with malondialdehyde and 4-hydroxyalkenals. Analytical applications to a colorimetric assay of lipid peroxidation, Chem Res Toxicol 11, 1176–1183.
Johansson, L. H. and Borg, L. A. (1988) A spectrophotometric method for determination of catalase activity in small tissue samples, Anal. Biochem 174, 331–336.
Wheeler, C. R., Salzman, J. A., Elsayed, N. M., Omaye, S. T., and Korte, D. W., Jr. (1990) Automated assays for superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase activity, Anal. Biochem 184, 193–199.
Spitz, D. R. and Oberley, L. W. (2001) Measurement of MnSOD and CuZnSOD Activity in Mammalian Tissue Homogenates, Current Protocols in Toxicology 7.5, 1–11.
Beauchamp, C. and Fridovich, I. (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels, Anal. Biochem 44, 276–287.
Weisiger, R. A. and Fridovich, I. (1973) Superoxide dismutase. Organelle specificity, J Biol Chem 248, 3582–3592.
Warren, G. L., Ingalls, C. P., Shah, S. J., and Armstrong, R. B. (1999) Uncoupling of in vivo torque production from EMG in mouse muscles injured by eccentric contractions, J Physiol 515 ( Pt 2), 609–619.
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Alway, S.E., Cutlip, R.G. (2012). Resistance Loading and Signaling Assays for Oxidative Stress in Rodent Skeletal Muscle. In: DiMario, J. (eds) Myogenesis. Methods in Molecular Biology, vol 798. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-343-1_11
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