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Oncolytic Measles Virus Retargeting by Ligand Display

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Oncolytic Viruses

Part of the book series: Methods in Molecular Biology ((MIMB,volume 797))

Abstract

Despite significant advances in recent years, treatment of metastatic malignancies remains a significant challenge. There is an urgent need for development of novel therapeutic approaches. Virotherapy approaches have considerable potential, and among them measles virus (MV) vaccine strains have emerged as a promising oncolytic platform. Retargeted MV strains deriving from the Edmonston vaccine lineage (MV-Edm) have shown comparable antitumor efficacy to unmodified strains against receptor expressing tumor cells with improved therapeutic index. Here, we describe the construction, rescue, amplification, and titration of fully retargeted MV-Edm derivatives displaying tumor specific receptor binding ligands on the viral surface in combination with H protein CD46 and SLAM entry ablating mutations.

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Author information

Authors and Affiliations

  1. Mayo Clinic, Rochester, MN, USA

    Pavlos Msaouel, Ianko D. Iankov, Cory Allen & Stephen J. Russell

  2. Department of Molecular Medicine, Mayo Clinic, Rochester, MN, USA

    Evanthia Galanis

Authors
  1. Pavlos Msaouel
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  2. Ianko D. Iankov
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  3. Cory Allen
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  4. Stephen J. Russell
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  5. Evanthia Galanis
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Corresponding author

Correspondence to Evanthia Galanis .

Editor information

Editors and Affiliations

  1. Jennerex Biotherapeutics, Inc., Market Street 1, San Francisco, 94105, California, USA

    David H. Kirn

  2. School of Medicine, Dept. Pathology & Immunology, Washington University, S. Euclid Ave. 660, St.Louis, 63110, Missouri, USA

    Ta-Chiang Liu

  3. Hillman Cancer Center, University of Pittsburgh, Centre Avenue 5117, Pittsburgh, 15213, Pennsylvania, USA

    Stephen H. Thorne

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Msaouel, P., Iankov, I.D., Allen, C., Russell, S.J., Galanis, E. (2012). Oncolytic Measles Virus Retargeting by Ligand Display. In: Kirn, D., Liu, TC., Thorne, S. (eds) Oncolytic Viruses. Methods in Molecular Biology, vol 797. Humana, Totowa, NJ. https://doi.org/10.1007/978-1-61779-340-0_11

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  • DOI: https://doi.org/10.1007/978-1-61779-340-0_11

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  • Publisher Name: Humana, Totowa, NJ

  • Print ISBN: 978-1-61779-339-4

  • Online ISBN: 978-1-61779-340-0

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