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Investigation of Acquired Resistance to EGFR-Targeted Therapies in Lung Cancer Using cDNA Microarrays

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Kinase Inhibitors

Part of the book series: Methods in Molecular Biology ((MIMB,volume 795))

Abstract

Clinical tools to accurately describe, evaluate, and predict an individual’s response to cancer therapy are a field-wide priority; in many advanced cancers, only 10–20% of individuals will have a clinical benefit from therapy, yet we treat the entire population. Furthermore, many therapies are initially effective, but lose effectiveness over time. Here we describe methods to derive in vitro models of resistance to EGFR tyrosine kinase inhibitors. We additionally describe approaches to characterize possible mechanisms of resistance by genomic and transcriptomic approaches.

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References

  1. Scaltriti, M., and Baselga, J. (2006) The epidermal growth factor receptor pathway: a model for targeted therapy. Clin. Cancer Res. 12, 5268–5272.

    Article  PubMed  CAS  Google Scholar 

  2. Lynch, T. J., Bell, D. W., Sordella, R., Gurubhagavatula, S., Okimoto, R. A., Brannigan, B. W., Harris, P. L., Haserlat, S. M., Supko, J. G., Haluska, F. G., Louis, D. N., Christiani, D. C., Settleman, J., and Haber, D. A. (2004) Activating mutations in the epidermal growth factor receptor underlying responsiveness of non-small-cell lung cancer to gefitinib. N. Engl. J. Med. 350, 2129–2139.

    Article  PubMed  CAS  Google Scholar 

  3. Choi, S. H., Mendrola, J. M., and Lemmon, M. A. (2007) EGF-independent activation of cell-surface EGF receptors harboring mutations found in gefitinib-sensitive lung cancer. Oncogene 26, 1567–1576.

    Article  PubMed  CAS  Google Scholar 

  4. Kumar, A., Petri, E. T., Halmos, B., and Boggon, T. J. (2008) Structure and clinical relevance of the epidermal growth factor receptor in human cancer. J. Clin. Oncol. 26, 1742–1751.

    Article  PubMed  CAS  Google Scholar 

  5. Shigematsu, H., and Gazdar, A. F. (2006) Somatic mutations of epidermal growth factor receptor signaling pathway in lung cancers. Int. J. Cancer 118, 257–262.

    Article  PubMed  CAS  Google Scholar 

  6. Engelman, J. A., Zejnullahu, K., Mitsudomi, T., Song, Y., Hyland, C., Park, J. O., Lindeman, N., Gale, C. M., Zhao, X., Christensen, J., Kosaka, T., Holmes, A. J., Rogers, A. M., Cappuzzo, F., Mok, T., Lee, C., Johnson, B. E., Cantley, L. C., and Janne, P. A. (2007) MET amplification leads to gefitinib resistance in lung cancer by activating ERBB3 signaling. Science 316, 1039–1043.

    Article  PubMed  CAS  Google Scholar 

  7. Jiang, N., Leach, L. J., Hu, X., Potokina, E., Jia, T., Druka, A., Waugh, R., Kearsey, M. J., and Luo, Z. W. (2008) Methods for evaluating gene expression from Affymetrix microarray datasets. BMC Bioinformatics 9, 284.

    Article  PubMed  Google Scholar 

  8. Kwak, E. L., Sordella, R., Bell, D. W., Godin-Heymann, N., Okimoto, R. A., Brannigan, B. W., Harris, P. L., Driscoll, D. R., Fidias, P., Lynch, T. J., Rabindran, S. K., McGinnis, J. P., Wissner, A., Sharma, S. V., Isselbacher, K. J., Settleman, J., and Haber, D. A. (2005) Irreversible inhibitors of the EGF receptor may circumvent acquired resistance to gefitinib. Proc. Natl. Acad. Sci. USA 102, 7665–7670.

    Article  PubMed  CAS  Google Scholar 

  9. Pao, W., Miller, V. A., Politi, K. A., Riely, G. J., Somwar, R., Zakowski, M. F., Kris, M. G., and Varmus, H. (2005) Acquired resistance of lung adenocarcinomas to gefitinib or erlotinib is associated with a second mutation in the EGFR kinase domain. PLoS Med. 2, e73.

    Article  PubMed  Google Scholar 

  10. Engelman, J. A., Mukohara, T., Zejnullahu, K., Lifshits, E., Borras, A. M., Gale, C. M., Naumov, G. N., Yeap, B. Y., Jarrell, E., Sun, J., Tracy, S., Zhao, X., Heymach, J. V., Johnson, B. E., Cantley, L. C., and Janne, P. A. (2006) Allelic dilution obscures detection of a biologically significant resistance mutation in EGFR-amplified lung cancer. J. Clin. Invest. 116, 2695–2706.

    Article  PubMed  CAS  Google Scholar 

  11. Oleykowski, C. A., Bronson Mullins, C. R., Godwin, A. K., and Yeung, A. T. (1998) Mutation detection using a novel plant endonuclease. Nucleic Acids Res. 26, 4597–4602.

    Article  PubMed  CAS  Google Scholar 

  12. Qiu, P., Shandilya, H., D’Alessio, J. M., O’Connor, K., Durocher, J., and Gerard, G. F. (2004) Mutation detection using Surveyor nuclease. Biotechniques 36, 702–707.

    PubMed  CAS  Google Scholar 

  13. Janne, P. A., Borras, A. M., Kuang, Y., Rogers, A. M., Joshi, V. A., Liyanage, H., Lindeman, N., Lee, J. C., Halmos, B., Maher, E. A., Distel, R. J., Meyerson, M., and Johnson, B. E. (2006) A rapid and sensitive enzymatic method for epidermal growth factor receptor mutation screening. Clin. Cancer Res. 12, 751–758.

    Article  PubMed  Google Scholar 

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Author information

Authors and Affiliations

  1. University of Southern California, Los Angeles, CA, USA

    Kian Kani & Parag Mallick

  2. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA

    Rafaella Sordella

  3. Stanford University, Stanford, CA, USA

    Parag Mallick

Authors
  1. Kian Kani
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  2. Rafaella Sordella
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  3. Parag Mallick
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Corresponding author

Correspondence to Parag Mallick .

Editor information

Editors and Affiliations

  1. LS für Proteomik und Bioanalytik, Technische Universität München, Emil Erlenmeyer Forum 5, Freising, 85354, Germany

    Bernhard Kuster

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Kani, K., Sordella, R., Mallick, P. (2012). Investigation of Acquired Resistance to EGFR-Targeted Therapies in Lung Cancer Using cDNA Microarrays. In: Kuster, B. (eds) Kinase Inhibitors. Methods in Molecular Biology, vol 795. Humana Press. https://doi.org/10.1007/978-1-61779-337-0_16

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  • DOI: https://doi.org/10.1007/978-1-61779-337-0_16

  • Published:

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-336-3

  • Online ISBN: 978-1-61779-337-0

  • eBook Packages: Springer Protocols

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