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Allosteric Mechanisms of G Protein-Coupled Receptor Signaling: A Structural Perspective

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Allostery

Part of the book series: Methods in Molecular Biology ((MIMB,volume 796))

Abstract

G protein-Coupled Receptors (GPCRs) use a complex series of intramolecular conformational changes to couple agonist binding to the binding and activation of cognate heterotrimeric G protein (Gαβγ). The mechanisms underlying this long-range activation have been identified using a variety of biochemical and structural approaches and have primarily used visual signal transduction via the GPCR rhodopsin and cognate heterotrimeric G protein transducin (Gt) as a model system. In this chapter, we review the methods that have revealed allosteric signaling through rhodopsin and transducin. These methods can be applied to a variety of GPCR-mediated signaling pathways.

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Authors and Affiliations

  1. Department of Biochemistry, Vanderbilt University Medical Center, Nashville, TN, USA

    Tarjani M. Thaker

  2. Department of Pharmacology, Vanderbilt University Medical Center, Nashville, TN, USA

    Ali I. Kaya, Anita M. Preininger & Heidi E. Hamm

  3. Departments of Biochemistry and Pharmacology, Vanderbilt University Medical Center, Nashville, TN, USA

    T. M. Iverson

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  1. Tarjani M. Thaker
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  2. Ali I. Kaya
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  3. Anita M. Preininger
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  4. Heidi E. Hamm
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  5. T. M. Iverson
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Correspondence to T. M. Iverson .

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  1. , Dept. Biochemistry & Molecular Biology, University of Kansas Medical Center, Rainbow Blvd. 3901, Kansas City, 66160, Kansas, USA

    Aron W. Fenton

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Thaker, T.M., Kaya, A.I., Preininger, A.M., Hamm, H.E., Iverson, T.M. (2012). Allosteric Mechanisms of G Protein-Coupled Receptor Signaling: A Structural Perspective. In: Fenton, A. (eds) Allostery. Methods in Molecular Biology, vol 796. Springer, New York, NY. https://doi.org/10.1007/978-1-61779-334-9_8

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  • DOI: https://doi.org/10.1007/978-1-61779-334-9_8

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