Site-Specific Incorporation of Unnatural Amino Acids as Probes for Protein Conformational Changes

  • Jennifer C. Peeler
  • Ryan A. MehlEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 794)


Site-specific in vivo incorporation of unnatural amino acids provides powerful tools for the study of protein interaction and dynamics. Here, we provide a protocol for the incorporation of six such UAA probes into a GFP reporter system, expressed in Escherichia coli from both arabinose and lactose-inducible expression plasmids using an autoinduction media.

Key words

Unnatural amino acid probe Autoinduction GFP reporter Amber codon suppression pDule2 plasmid 


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of ChemistryFranklin & Marshall CollegeLancasterUSA

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