Carbon–Carbon Bond-Forming Enzymes for the Synthesis of Non-natural Amino Acids

  • Pere ClapésEmail author
  • Jesús Joglar
  • Mariana Gutiérrez
Part of the Methods in Molecular Biology book series (MIMB, volume 794)


An enzymatic methodology for the preparation of β-hydroxy-α-amino acid derivatives is described. The method consists of the stereoselective aldol addition reaction of glycine to N-Cbz-amino aldehydes furnishing 3-hydroxy-2,4-diaminobutyric derivatives.

Key words

Lyases Biotransformation Aldol reaction Low specific l-allo-threonine aldolases Serine hydroxymethyl transferase 


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Pere Clapés
    • 1
    Email author
  • Jesús Joglar
    • 2
  • Mariana Gutiérrez
    • 2
  1. 1.Department of Chemical Biology and Molecular ModelingInstituto de Química Avanzada de Cataluña IQAC-CSICBarcelonaSpain
  2. 2.Department of Chemical Biology and Molecular ModelingInstituto de Química Avanzada de Cataluña-CSICBarcelonaSpain

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