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Assay of Amino Acid Racemases

  • Masumi Katane
  • Masae Sekine
  • Hiroshi HommaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 794)

Abstract

d-Amino acids play important physiological roles in the mammalian body. Recent investigations revealed that, in mammals, d-amino acids are synthesized from their corresponding l-enantiomers via amino acid racemase. This article describes a method used to measure amino acid racemase activity by high-performance liquid chromatography (HPLC). The assay involves fluorogenic chiral derivatization of amino acids with a newly developed reagent, and enantioseparation of d- and l-amino acid derivatives by HPLC. The method is accurate and reliable, and can be automated using a programmable autosampling injector.

Key words

d-Amino acid Racemase Enantioseparation Chiral derivatization High-performance liquid chromatography 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of Pharmaceutical Life SciencesKitasato UniversityMinatokuJapan

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