Preparation and Assay of Recombinant Serine Racemase

  • Florian Baumgart
  • Clara Aicart-Ramos
  • Ignacio Rodriguez-CrespoEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 794)


Serine racemase is a glial and neuronal enzyme that reversibly converts l-serine to d-serine, an endogenous co-agonist of N-methyl-d-aspartate receptor type glutamate receptors (NMDARs). Here we present methods to recombinantly express and purify serine racemase in bacteria and two complementary ways to determine d-serine levels in unknown samples. Furthermore, a detailed protocol of serine racemase activity assays is described that can be used to screen for activators and inhibitors in vitro.

Key words

Serine racemase d-Serine PLP enzymes Racemization 



This work was supported by Grants BFU2006-05395 and BFU2009-10442 from the Spanish MICINN.


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Florian Baumgart
    • 1
  • Clara Aicart-Ramos
    • 1
  • Ignacio Rodriguez-Crespo
    • 1
    Email author
  1. 1.Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias QuímicasUniversidad ComplutenseMadridSpain

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