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Neurodegeneration pp 363–376Cite as

Imaging Presynaptic Exocytosis in Corticostriatal Slices

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 793))

Abstract

Optical imaging is a valuable tool for investigating alterations in membrane turnover and vesicle trafficking. Established techniques can easily be adapted to study the mechanisms of synaptic dysfunction in models of neuropsychiatric disorders and neurodegenerative diseases, such as drug addiction, Parkinsonism, and Huntington’s disease. Fluorescent endocytic tracers, including FM1-43, have been used to optically monitor synaptic vesicle fusion and measure synaptic function in various preparations, including chromaffin cells, dissociated cell cultures, and brain slices. In this chapter, we describe a technique that provides a direct measure of pathway-specific exocytosis from glutamatergic corticostriatal terminals.

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Acknowledgments

This work was supported by DA07418, Picower and Parkinson’s Disease Foundations (DS) and by NS052536, NS060803, HD02274, University of Washington Vision Research Center and Children’s Hospital, Seattle, WA (NSB).

Author information

Authors and Affiliations

  1. Department of Neurology, Columbia University, New York, NY, USA

    Minerva Y. Wong & David Sulzer

  2. Department of Pharmacology, Columbia University, New York, NY, USA

    David Sulzer

  3. Department of Neurology, University of Washington and Seattle Children’s Hospital, Seattle, WA, USA

    Nigel S. Bamford

  4. Department of Pediatrics and Psychology, University of Washington and Seattle Children’s Hospital, Seattle, WA, USA

    Nigel S. Bamford

  5. The Graduate Program in Neurobiology and Behavior, University of Washington and Seattle Children’s Hospital, Seattle, WA, USA

    Nigel S. Bamford

  6. Center on Human Development and Disability, University of Washington and Seattle Children’s Hospital, Seattle, WA, USA

    Nigel S. Bamford

Authors
  1. Minerva Y. Wong
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  2. David Sulzer
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  3. Nigel S. Bamford
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Corresponding author

Correspondence to Nigel S. Bamford .

Editor information

Editors and Affiliations

  1. Weill Medical College, Dept. Neurology & Neuroscience, Cornell University, East 68th St. 525, New York, 10065, New York, USA

    Giovanni Manfredi

  2. York Avenue 1300, New York, 10065, New York, USA

    Hibiki Kawamata

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Wong, M.Y., Sulzer, D., Bamford, N.S. (2011). Imaging Presynaptic Exocytosis in Corticostriatal Slices. In: Manfredi, G., Kawamata, H. (eds) Neurodegeneration. Methods in Molecular Biology, vol 793. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-328-8_24

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  • DOI: https://doi.org/10.1007/978-1-61779-328-8_24

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-327-1

  • Online ISBN: 978-1-61779-328-8

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