Abstract
Enumeration of antigen-specific cells after vaccination is one of the prime immunological parameters determined when developing vaccines. Due to their exquisite sensitivity (limits of detection can be below 1/100,000 cells), ELISPOT assays are therefore an important tool in vaccine development programs. This is particularly the case for vaccines against diseases that require protective cell-mediated immunity, such as tuberculosis. This chapter describes ELISPOT assays detecting bovine IFN-γ.
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References
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Acknowledgements
The authors’ work was supported by the Department for Environment, Food and Rural Affairs, the UK.
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Vordermeier, M., Whelan, A.O. (2012). ELISPOT Assays to Enumerate Bovine IFN-γ-Secreting Cells for the Development of Novel Vaccines Against Bovine Tuberculosis. In: Kalyuzhny, A. (eds) Handbook of ELISPOT. Methods in Molecular Biology, vol 792. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-325-7_17
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DOI: https://doi.org/10.1007/978-1-61779-325-7_17
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Publisher Name: Humana Press, Totowa, NJ
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