Abstract
Compared with conventional gel-based techniques, such as gel electrophoresis, which are routinely used for bioseparation in biology and biomedical laboratories, nanofluidic devices with regular engineered sieving structures offer the potential for faster separation, better resolution, higher throughput, and more convenient sample recovery. Here, we detail the fabrication process of a two-dimensional nanofluidic filter array device and its implementation for rapid continuous-flow separation of biomolecules such as proteins.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Scopes, R.K. (1993) Protein Purification, Principles and Practice. Springer-Verlag, New York.
Goodsell, D.S. (2004) Bionanotechnology, Lessons from Nature. Wiley-Liss, Hoboken, New Jersey.
Tegenfeldt, J.O., Prinz, C., Cao, H., et al. (2004) Micro- and nanofluidics for DNA analysis. Anal Bioanal Chem 378, 1678–1692.
Han, J., Fu, J., Schoch, R.B. (2008) Molecular Sieving Using Nanofilters: Past, Present and Future. Lab Chip 8, 23–33.
Fu, J., Mao, P., Han, J. (2008) Artificial molecular sieves and filters: a new paradigm for biomolecule separation. Trends Biotechnol 26, 311–320.
Han, J., Craighead, H.G. (2000) Separation of Long DNA Molecules in a Microfabricated Entropic Trap Array. Science 288, 1026–1029.
Huang, L.R., Tegenfeldt, J.O., Kraeft, J.J., et al. (2002) A DNA prism for high-speed continuous fractionation of large DNA molecules. Nat Biotechnol 20, 1048–1051.
Fu, J., Schoch, R.B., Stevens, A.L., et al. (2007) A patterned anisotropic nanofluidic sieving structure for continuous-flow separation of DNA and proteins. Nat Nanotechnol 2, 121–128.
Fu, J., Mao, P., Han, J. (2009) Continuous-flow bioseparation using microfabricated anisotropic nanofluidic sieving structures. NatProtoc 4, 1681–1698.
Eijkel, J.C.T., van den Berg, A. (2006) Nanotechnology for membranes, filters and sieves: A series of mini-reviews covering new trends in fundamental and applied research, and potential applications of miniaturised technologies. Lab Chip 6, 19–23.
Pamme, N. (2007) Continuous flow separations in microfluidic devices. Lab Chip 7, 1644–1659.
Righetti, P.G., Castagna, A., Herbert, B., et al. (2003) Prefractionation techniques in proteome analysis. Proteomics 3, 1397–1407.
Madou, M. (1997) Fundamentals of microfabrication. CRC Press, Boca Raton, Florida.
Acknowledgments
The authors wish to acknowledge financial support from the Department of Mechanical Engineering of the University of Michigan. The authors also extend their appreciation to J. Yoo for his contribution in the experimental setup and to H. Bow for helpful discussions. The MIT Microsystems Technology Laboratories is acknowledged for support for the cleanroom microfabrication.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2011 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Mao, P., Fu, J. (2011). Nanofluidic Devices for Rapid Continuous-Flow Bioseparation. In: Toms, S., Weil, R. (eds) Nanoproteomics. Methods in Molecular Biology, vol 790. Humana Press. https://doi.org/10.1007/978-1-61779-319-6_10
Download citation
DOI: https://doi.org/10.1007/978-1-61779-319-6_10
Published:
Publisher Name: Humana Press
Print ISBN: 978-1-61779-318-9
Online ISBN: 978-1-61779-319-6
eBook Packages: Springer Protocols