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Epigenetics Protocols pp 179–193Cite as

A Chromatin Immunoprecipitation Protocol for Small Cell Numbers

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 791))

Abstract

Chromatin immunoprecipitation (ChIP) is a widely used technique to get a snap-shot of protein–DNA interactions in cells. ChIP has notably been used for mapping the location of modified histones, transcription factors, or chromatin remodeling enzymes in the genome, often in relation to transcription or differentiation. Conventional ChIP protocols however, have for a long time required large numbers of cells, which has limited the applicability of ChIP to rare or small cell samples. In recent years, ChIP assays for small cell numbers (in the 10,000–100,000 range) have been recently reported by us and others. This chapter describes a micro (μ)ChIP procedure for multiple parallel ChIPs from a single chromatin batch from 1,000 cells.

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Acknowledgments

Dr. John Arne Dahl is thanked for setting up the protocol in the laboratory. Our work is supported by the Research Council of Norway, the Norwegian Cancer Society, and the University of Oslo.

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Authors and Affiliations

  1. University of Oslo, Institute of Basic Medical Sciences & Norwegian Center for Stem Cell Research, Oslo, Norway

    Philippe Collas

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  1. Philippe Collas
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Correspondence to Philippe Collas .

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Editors and Affiliations

  1. Dept. Biology, University of Alabama, Birmingham, University Blvd. 1300, Birmingham, 35294-1170, Alabama, USA

    Trygve O. Tollefsbol

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Collas, P. (2011). A Chromatin Immunoprecipitation Protocol for Small Cell Numbers. In: Tollefsbol, T. (eds) Epigenetics Protocols. Methods in Molecular Biology, vol 791. Humana Press. https://doi.org/10.1007/978-1-61779-316-5_14

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  • DOI: https://doi.org/10.1007/978-1-61779-316-5_14

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-315-8

  • Online ISBN: 978-1-61779-316-5

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