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6-OHDA Toxin Model in Mouse

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Animal Models of Movement Disorders

Part of the book series: Neuromethods ((NM,volume 61))

Abstract

The unilateral 6-hydroxydopamine mouse model has received considerable attention of late as a model complementary to the hemi-parkinsonian rat. Although both species are similar in nature, there are significant differences between the two when conducting stereotaxic surgery, such as anaesthesia maintenance, technical procedure and differences in lesion co-ordinates. In the present chapter, we therefore discuss detailed methods, problems and suitability of mouse lesion techniques. Mice are also more prone to high post-lesion mortality rates and weight loss, therefore requiring more vigilant care. We describe basic behavioural tests that determine the level of dopaminergic cell death in mice, namely: drug-induced and spontaneous rotations, elevated beam test, staircase test, cylinder test, corridor, and rotarod for assessing lesion-induced deficits in mice. A number of other tests used to assess the rat model cannot however be adapted for use in the mouse.

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Acknowledgments

Our experiments in this field are supported by grants from the UK Medical Research Council, the UK Biotechnology and Biological Science Research Council, and the European Union Seventh Framework programme.

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Authors and Affiliations

  1. The Brain Repair Group, School of Biosciences, Cardiff University, Cardiff, Wales, UK

    Gaynor A. Smith & Andreas Heuer

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  1. Gaynor A. Smith
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  2. Andreas Heuer
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Correspondence to Gaynor A. Smith .

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Editors and Affiliations

  1. , Welsh School of Pharmacology, Cardiff University, Edward VII Avenue, Cardiff, CF10 3NB, United Kingdom

    Emma L. Lane

  2. School of Biosciences, Brain Repair Group, Cardiff University, Museum Avenue, Cardiff, CF10 3AX, United Kingdom

    Stephen B. Dunnett

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Smith, G.A., Heuer, A. (2011). 6-OHDA Toxin Model in Mouse. In: Lane, E., Dunnett, S. (eds) Animal Models of Movement Disorders. Neuromethods, vol 61. Humana Press. https://doi.org/10.1007/978-1-61779-298-4_14

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  • DOI: https://doi.org/10.1007/978-1-61779-298-4_14

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-297-7

  • Online ISBN: 978-1-61779-298-4

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