Abstract
We provide here a protocol for the preparation of cap-analysis gene expression (CAGE) libraries, which allows for measuring the expression of eukaryotic capped RNAs and simultaneously map the promoter regions. The presented protocol simplifies the previously published ones and moreover produces tags that are 27 nucleotides long, which facilitates mapping to the genome. The protocol takes less than 5 days to complete and presents a notable improvement compared to previously published versions.
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Acknowledgments
This work was founded by a Research Grant for RIKEN Omics Science Center from MEXT and by the National Human Genome Research Institute grants U54 HG004557. We thank all the colleagues at the OSC for the precious feedbacks during the development of the methodology.
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Takahashi, H., Kato, S., Murata, M., Carninci, P. (2012). CAGE (Cap Analysis of Gene Expression): A Protocol for the Detection of Promoter and Transcriptional Networks. In: Deplancke, B., Gheldof, N. (eds) Gene Regulatory Networks. Methods in Molecular Biology, vol 786. Humana Press. https://doi.org/10.1007/978-1-61779-292-2_11
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DOI: https://doi.org/10.1007/978-1-61779-292-2_11
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