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MicroRNA Expression Analysis: Techniques Suitable for Studies of Intercellular and Extracellular MicroRNAs

  • Erica HennessyEmail author
  • Lorraine O’Driscoll
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 784)

Abstract

MicroRNAs, the class of small ribo-regulators, have been implicated in the regulation of a range of different biological processes, including development and differentiation, proliferation, and cell death. Only for a small fraction of identified microRNAs has a function been elucidated; therefore, a great deal of research remains to be performed to fully understand the role and implications of microRNAs.

This chapter discusses protocols for the isolation of microRNAs, reverse transcription, PCR, and large scale profiling using TaqMan low density miRNA arrays for analysis of microRNA expression levels.

Key words

MicroRNA miRNA TaqMan low density microRNA array Megaplex RT primers 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.National Institute for Cellular BiotechnologyDublin City UniversityDublinIreland
  2. 2.School of Pharmacy and Pharmaceutical SciencesTrinity College DublinDublinIreland

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